We Universal Biotechnology has augmented its extensive catalog of SARS-CoV-2-related antibodies and reagents with the production of recombinant antibodies targeting the viral spike and nucleocapsid proteins. The spike S1 recombinant antibodies demonstrate specificity for SARS-CoV-2 S1 with no cross-reactivity to SARS-CoV or MERS-CoV spike proteins. Clones [HL1] and [HL6] in particular exhibit high affinity for mammalian-expressed SARS-CoV-2 spike protein with EC50 values of 0.117 nM and 0.251 nM, respectively. Importantly, both antibodies perform exceptionally well when paired with GeneTex’s mouse monoclonal SARS-CoV antibody [1A9] for sandwich ELISA assays. Though there can be high homology among various regions of different coronavirus nucleocapsid proteins, GeneTex’s SARS-CoV-2 nucleocapsid antibody [HL344] is specific for SARS-CoV-2 with no cross-reactivity to MERS-CoV or SARS-CoV nucleocapsid. All eight recombinant nucleocapsid clones display high affinity for mammalian-expressed full-length SARS-Cov-2 nucleocapsid protein (EC50 values ranging from 0.006-0.074 nM), among which a SARS-CoV-2 nucleocapsid recombinant antibody pair (GTX635685; GTX635712) designed for sandwich ELISA (sELISA; GTX500042) reaching a superior sensitivity of ~3 pg/mL of recombinant nucleocapsid protein.
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