For best results, it is essential to optimize all system components including sample amount, antibody concentrations and the membrane and blocking reagents. SuperLumia ECL Reagent is extremely sensitive and requires much less sample and antibodies than most commercially available substrates.
Features & Benefits
Abbkine SuperLumia ECL Plus HRP Substrate Kit is a value-priced, entry-level peroxidase substrate for enhanced chemiluminescence (ECL) that directly replaces costlier products without the need to re-optimize conditions.
Mix the two substrate components at a 1:1 ratio to prepare the substrate working solution.2.Suggest 0.1-0.2ml of SuperLumia ECL Plus working solution for 1 cm2 size of blot membrane. 3.Incubate blot 1-5 minutes in SuperLumia ECL Plus Substrate working solution.4.Drain excess reagent. Cover blot with clear plastic wrap.5.Expose blot to X-ray film.
Stable for one year at 4-8°C from date of shipment. Exposure to the sun or any other intense light can harm the working solution. Short-term exposure to typical laboratory lighting will not harm the working solution.