GScript First-Strand Synthesis Kit
The GScript First-Strand Synthesis Kit is a recombinant M-MLV RTase expressed in E. coli and purified to homogeneity. It has a lower RNase H activity and a high thermal stability. The enzyme is widely used to synthesize first-strand cDNA at temperatures up to 55°C with increased specificity, higher yields of cDNA, and more full-length product than other reverse transcriptases. It can generate cDNA from 100 bp to 12 Kb. The GScript RTase has a special activity with its secondary structure, rendering stabilization during the reverse transcription reaction.
Size: 50 Reactions
- Store all components at -20°C (non-frost-free).
- Thaw 5X 1st strand buffer and DTT (0.1 M) at the room temperature just prior to use and refreeze immediately.
- Thaw the dNTP mix (10 mM) and Oligo(dT)20 on ice prior to use and refreeze immediately.
|Oligo(dT)20 (50 μM)||50 μl|
|5X 1st strand buffer||250 μl|
|DTT (0.1 M)||100 μl|
|dNTP mix (10 mM)||50 μl|
|GScript RTase (200 U/ μl)||50 μl|
The quality of the GScript First-Strand Synthesis Kit is tested on a lot-to-lot basis Size: 50 Reactionsto ensure consistent product quality.