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Manufacturer: Elabscience

Blood protein can be precipitated with protein precipitator, and enzyme activity will be destroyed, which can prevent the formation of free ammonia in vitro. Most interfering color substances were removed at the same time, indigo was formed in non-protein filtrate by Berthelot reaction, and the color depth was proportional to the content of blood ammonia. Blood ammonia content can be determined by comparing with standard solution.

Manufacturer: Elabscience

β-galactosidase activated by sodium ions can catalyze glycoside analogues to produce glycoside and o-nitrophenol. The increase of absorbance is determined at 405 nm, and the content of sodium ion is calculated indirectly.

Manufacturer: Elabscience

Glycerol is transformed by enzyme to produce hydrogen peroxide, which is catalyzed by oxidase in the presence of 4-amino-antipyrprid and phenol to produce red quinones, the color of which is proportional to the content of glycerol.

Manufacturer: Elabscience

Using NAD+ as H+ receptor, D-lactate dehydrogenase (LDH) catalyzes the reaction of D-lactic acid and NAD+ to generate pyruvic acid and NADH respectively. NBT is reduced to a kind of purple compound during the reaction. Measure the OD value at 530 nm, and the concentration of D-lactic acid can be calculated.

Manufacturer: Elabscience

Creatine Kinase catalyzes adenosine triphosphate and creatine to produce creatine phosphate, then detected by phosphomolybdic acid colorimetry.

Manufacturer: Elabscience

DPPH is a synthetic organic free radical that can be used to evaluate the antioxidant activity of antioxidants. Its single electron has the maximum absorption at 525 nm, and the DPPH radical scavenging ability of the sample can be calculated by measuring the change of OD value before and after the addition of antioxidants.

Manufacturer: Elabscience

Ascorbate Peroxidase (APX) can catalyze the reaction between ascorbic acid (ASA) and hydrogen peroxide (H2O2), and ASA can be oxidized to monodehydroascorbic acid (MDASA). The absorbance of solution at 290 nm will decline as the oxidation of ASA. The APX activity can be calculated by detecting the decrease of A290.

Manufacturer: Elabscience

Fe3+ can be deoxidized to Fe2+ by VE with ferroin existing. Fe2+ can react with phenanthroline and form pink compound under certain condition. VE content can be calculated by measuring the OD value at 532 nm.

Manufacturer: Elabscience

With S-NAD+ as hydrogen receptor, 3α-hydroxy steroid dehydrogenase catalyzed the dehydrogenation of bile acids to produce 3-ketone steroids, transforming S-NAD+ into S-NADH. Meanwhile, NADH was used as hydrogen donor. 3α-hydroxy steroid dehydrogenase catalyzed the production of bile acids from 3-ketone steroids. Through the enzyme cycle reaction, S-NADH is continuously generated, which has the maximum absorption peak at 405 nm. Measure the OD value at 405 nm and the changes of absorbance is proportional to the concentration of bile acid.