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Manufacturer: Elabscience

Creatine Kinase catalyzes adenosine triphosphate and creatine to produce creatine phosphate, then detected by phosphomolybdic acid colorimetry.

Manufacturer: Elabscience

DPPH is a synthetic organic free radical that can be used to evaluate the antioxidant activity of antioxidants. Its single electron has the maximum absorption at 525 nm, and the DPPH radical scavenging ability of the sample can be calculated by measuring the change of OD value before and after the addition of antioxidants.

Manufacturer: Elabscience

Ascorbate Peroxidase (APX) can catalyze the reaction between ascorbic acid (ASA) and hydrogen peroxide (H2O2), and ASA can be oxidized to monodehydroascorbic acid (MDASA). The absorbance of solution at 290 nm will decline as the oxidation of ASA. The APX activity can be calculated by detecting the decrease of A290.

Manufacturer: Elabscience

Fe3+ can be deoxidized to Fe2+ by VE with ferroin existing. Fe2+ can react with phenanthroline and form pink compound under certain condition. VE content can be calculated by measuring the OD value at 532 nm.

Manufacturer: Elabscience

With S-NAD+ as hydrogen receptor, 3α-hydroxy steroid dehydrogenase catalyzed the dehydrogenation of bile acids to produce 3-ketone steroids, transforming S-NAD+ into S-NADH. Meanwhile, NADH was used as hydrogen donor. 3α-hydroxy steroid dehydrogenase catalyzed the production of bile acids from 3-ketone steroids. Through the enzyme cycle reaction, S-NADH is continuously generated, which has the maximum absorption peak at 405 nm. Measure the OD value at 405 nm and the changes of absorbance is proportional to the concentration of bile acid.

Manufacturer: Elabscience

Diamine oxidase can catalyse amine substances to produce hydrogen peroxide, and hydrogen peroxide can react with the chromogenic substance to produce chromogenic substance, which has a characteristic absorption peak at 460 nm. The activity of diamine oxidase can be calculated by measuring the change rate of absorbance per unit time.

Manufacturer: Elabscience

Phosphotungstic acid can be reduced by Cys and form tungsten blue, which has an absorption peak at 600 nm. Cys content can be calculated with the absorbance at 600 nm.

Manufacturer: Procell System

DMEM (Dulbecco's Modified Eagle Medium) was developed on the basis of MEM medium. Compared with MEM medium, the content of amino acid increased by 2 times, the content of vitamin increased by 4 times, and the content of non-essential amino acid, trace iron ion and sodium pyruvate were increased by 4 times. The glucose content of DMEM medium was originally designed as 1000 mg/L (low Glucose type), and then developed into 4500 mg/L (high Glucose type), which has been widely used in cell culture. DMEM (High glucose) was widely used in fast growth, low adhesion cells, hybridoma myeloma cells, clone cells, DNA transfected transformation cells, various primary virus host cells, single cell culture and vaccine production. DMEM (High glucose) contains many kinds of amino acids, vitamins, inorganic salts and other ingredients for cell culture, but does not contain protein, lipids or any growth factors, so the product should be used with serum or serum-free additives.

Manufacturer: Procell System

Balanced Salt Solution (Physiological Solution) have the properties of buffer solution (regulate pH), normal saline (maintain osmotic pressure) and culture medium (provide nutrition). It can meet the basic needs of survival and metabolism of tissues, organs or cells in vitro. A small amount of phenolic red was added to some equilibrium salt solutions to indicate the pH change of the solution. Phosphate Buffered Saline (PBS) is one of the most widely used equilibrium salt solutions in biochemistry. The main components are Na2HPO4、KH2PO4、NaCl and KCl. PBS buffers are often used to rinse tissue or cells, to transport cells or tissues, to prepare other reagents, to dilute the cells when counting the cells.