Cell Culture

Manufacturer: Elabscience

Normal saline refers to the sodium chloride solution commonly used in physiological experiments or clinical practice, whose osmotic pressure is equal to that of animals or human body. It is commonly used as a fluid supplement and is also often used in vitro. Different types of animals require different concentrations of normal saline. Mammals use 0.9% normal saline, birds use 0.75% normal saline, and amphibians use 0.65% normal saline.

Manufacturer: Elabscience

RPMI-1640 is an improved McCoy's 5A medium, which uses bicarbonate buffer system. RPMI-1640 medium was originally designed for lymphocyte culture. Nowadays, it has been widely used in the culture of normal cells and cancer cells, especially suspension cells, which is one of the most widely used media. RPMI-1640 medium contains many kinds of amino acids, vitamins, inorganic salts and other ingredients for cell culture, but does not contain protein, lipids or any growth factors. Therefore, the product should be used serum or serum-free additives.

Manufacturer: Elabscience

NK-92MI Cell Complete Medium is a ready-to-use medium that can be directly used for the culture of NK-92MI cells, which is carefully designed and optimized by Pricella's R&D team based on MEMα basal medium. This high-quality complete medium has been strictly verified by NK-92MI cells which could promote cell proliferation and maintain cell properties.

Manufacturer: Elabscience

MEMα was developed on the basis of MEM medium. Compared with MEM medium, NEAA, sodium pyruvate, zinc sulfate, VB12, biotin and ascorbic acid were added. It is widely used in suspension and adherent cell culture of various mammals. MEMα medium without nucleoside and deoxynucleoside is often used as medium for DG44 and other DHFR- cells. This product contains many kinds of amino acids, vitamins, inorganic salts and other ingredients for cell culture, but does not contain protein, lipids or any growth factors. Therefore, the product should be used with serum or serum-free additives.

Manufacturer: Elabscience

L-alanyl-L-glutamine is an advanced cell culture additive and as a substitute for L-glutamine. L-glutamine is a necessary nutrient element for cells. But it is unstable in solution and can be degraded spontaneously to form ammonia and pyroglutamic acid and ammonia is harmful to cells. L-alanyl-L-glutamine is stable in aqueous solution and does not degrade spontaneously. The mechanism used by the cell is that the cell gradually releases a peptidase into the culture medium during cell culture. The peptidase can hydrolyze L-alanyl-L-glutamine into L-alanine and L-glutamine. L-alanine and L-glutamine can be absorbed and utilized by cells. This not only increases the utilization rate of L-glutamine, but also does not produce excess ammonia, which is more beneficial to cell growth.

Manufacturer: Elabscience

The DMEM/F12 medium is based on the DMEM medium, adding more nutritious elements from F12 medium, which contains a variety of trace elements, and is widely used in the culture of many mammalian cells. At the same time, DMEM/F12 medium is often used as the basis for the development of serum-free medium. It is also suitable for mammalian cell culture and clone density culture under low serum content. This product contains many kinds of amino acids, vitamins, inorganic salts and other ingredients for cell culture, but does not contain protein, lipids or any growth factors. Therefore, the product should be used with serum or serum-free additives.

Manufacturer: Elabscience

NCTC clone 929 Cell Complete Medium is a ready-to-use medium that can be directly used for the culture of NCTC clone 929 cells, which is carefully designed and optimized by Pricella's R&D team based on MEM, with NEAA basal medium. This high-quality complete medium has been strictly verified by NCTC clone 929 cells which could promote cell proliferation and maintain cell properties.

Manufacturer: Elabscience

MCDB is a low-protein and serum-free culture medium designed for specific cells. MCDB 131 is a kind of MCDB medium designed and developed by Kendler and Ham. It was originally used for culturing human microvascular endothelial cells (HMVEC). In addition to the use of MCDB 131 medium for endothelial cells, it can also be used for other cell types, such as liver cells, smooth muscle cells, and cardiomyocytes. MCDB 131 medium is not used in traditional medium because it contains trace elements, putrescine, adenine, thymine and higher concentrations of amino acids and vitamins. It is often used with EGF, hydrocortisone, glutamine and low-concentration serum together, and the added concentration of serum must be determined by cell type.

Manufacturer: Elabscience

MCF7 [MCF-7] Cell Complete Medium is a ready-to-use medium that can be directly used for the culture of MCF7 [MCF-7] cells, which is carefully designed and optimized by Pricella's R&D team based on MEM, with NEAA basal medium. This high-quality complete medium has been strictly verified by MCF7 [MCF-7] cells which could promote cell proliferation and maintain cell properties.