Cell Culture

Manufacturer: Elabscience

MDA-MB-231 Cell Complete Medium is a ready-to-use medium that can be directly used for the culture of MDA-MB-231 cells, which is carefully designed and optimized by Pricella's R&D team based on DMEM basal medium. This high-quality complete medium has been strictly verified by MDA-MB-231 cells which could promote cell proliferation and maintain cell properties.

Manufacturer: Elabscience

RPMI-1640 is an improved McCoy's 5A medium, which uses bicarbonate buffer system. RPMI-1640 medium was originally designed for lymphocyte culture. Nowadays, it has been widely used in the culture of normal cells and cancer cells, especially suspension cells, which is one of the most widely used media. RPMI-1640 medium contains many kinds of amino acids, vitamins, inorganic salts and other ingredients for cell culture, but does not contain protein, lipids or any growth factors. Therefore, the product should be used serum or serum-free additives.

Manufacturer: Elabscience

3T3-L1 Cell Complete Medium is a ready-to-use medium that can be directly used for the culture of 3T3-L1 cells, which is carefully designed and optimized by Pricella's R&D team based on DMEM basal medium. This high-quality complete medium has been strictly verified by 3T3-L1 cells which could promote cell proliferation and maintain cell properties.

Manufacturer: Elabscience

HT22 Cell Complete Medium is a ready-to-use medium that can be directly used for the culture of HT22 cells, which is carefully designed and optimized by Pricella's R&D team based on DMEM basal medium. This high-quality complete medium has been strictly verified by HT22 cells which could promote cell proliferation and maintain cell properties.

Manufacturer: Elabscience

HEPES is all known as N-2-hydroxyethylpiperazine-N-2-ethane sulfonic acid, is a commonly used biological buffer. pKa (25℃) is 7.5 and an effective buffer range of pH is 6.8-8.2. The greatest advantage of HEPES is that to maintain the pH range of a solution for a longer period of time in an open environment. HEPES cannot pass through biofilms. It is a chemical and enzymatic stable substance with limited effect on biochemical reactions, and the absorption of visible and ultraviolet light is extremely low. Therefore, it is generally considered that HEPES is an excellent biological buffer, and there is no toxic effect to the cells. The working concentration is 10-50 mM and the final concentration for cell culture is 10 mM.

Manufacturer: Elabscience

DMEM (Dulbecco's Modified Eagle Medium) was developed on the basis of MEM medium. Compared with MEM medium, the content of amino acid increased by 2 times, the content of vitamin increased by 4 times, and the content of non-essential amino acid, trace iron ion and sodium pyruvate were increased by 4 times. The glucose content of DMEM medium was originally designed as 1000 mg/L (low Glucose type), and then developed into 4500 mg/L (high Glucose type), which has been widely used in cell culture. DMEM (High glucose) was widely used in fast growth, low adhesion cells, hybridoma myeloma cells, clone cells, DNA transfected transformation cells, various primary virus host cells, single cell culture and vaccine production. DMEM (High glucose) contains many kinds of amino acids, vitamins, inorganic salts and other ingredients for cell culture, but does not contain protein, lipids or any growth factors, so the product should be used with serum or serum-free additives.

Manufacturer: Elabscience

H9c2(2-1) Cell Complete Medium is a ready-to-use medium that can be directly used for the culture of H9c2(2-1) cells, which is carefully designed and optimized by Pricella's R&D team based on DMEM basal medium. This high-quality complete medium has been strictly verified by H9c2(2-1) cells which could promote cell proliferation and maintain cell properties.

Manufacturer: Elabscience

Sodium Pyruvate, the most common pyruvate, it is an intermediate product of glycolysis. It can directly enter and exit cells. It is a necessary nutrient for cell metabolism. In the absence of glucose, it can be used as a source of energy for cells. It is one of the commonly used additives in cell culture. Although cells can produce sodium pyruvate, some cells require additional addition of sodium pyruvate during in vitro culture. A certain amount of sodium pyruvate can also be added during clonal culture or low level of serum concentration, and sodium pyruvate also can reduce phototoxic effects caused by fluorescent substances.

Manufacturer: Elabscience

MC3T3-E1 Subclone 14 Cell Complete Medium is a ready-to-use medium that can be directly used for the culture of MC3T3-E1 Subclone 14 cells, which is carefully designed and optimized by Pricella's R&D team based on MEMα basal medium. This high-quality complete medium has been strictly verified by MC3T3-E1 Subclone 14 cells which could promote cell proliferation and maintain cell properties.