Cell Culture

Manufacturer: Elabscience

Caco-2 Cell Complete Medium is a ready-to-use medium that can be directly used for the culture of Caco-2 cells, which is carefully designed and optimized by Pricella's R&D team based on MEM, with NEAA basal medium. This high-quality complete medium has been strictly verified by Caco-2 cells which could promote cell proliferation and maintain cell properties.

Manufacturer: Elabscience

L-glutamine is an essential element in cell culture that is not only an energy source of the cells, but also an important substance involved in protein synthesis and nucleic acid metabolism. The deficiency of L-glutamine will lead to poor cell growth and even death. L-glutamine is unstable in solution and easy to degrade spontaneously, so it is necessary to supplement a certain amount of L-glutamine in culture medium to meet the needs of cell growth after a period of time or when cells grow badly.

Manufacturer: Elabscience

M199 (Medium 199) originally used in nutritional studies of chicken embryo fibroblasts, it has been widely used in the culture of various animal cells, including some non-mammalian cells. M199 is especially suitable for the culture of untransformed cells, rat pancreatic epithelial cells and mouse lens tissues, also used in virology and vaccine production. Compared with other basic medium, M199 contains unique ingredients, including adenine, adenosine, hypoxanthine, thymine, and other vitamins. There are two kinds of equilibrium salt (earle's salt and hank's salt) in M199. Earle's salt is often used in CO2 environment, hank's salt is used in non-CO2 environment. This product contains many kinds of amino acids, vitamins, inorganic salts and other ingredients for cell culture, but does not contain protein, lipids or any growth factors. Therefore, the product should be used with serum or serum-free additives.

Manufacturer: Elabscience

MEM medium (Minimum Essential Medium) was developed on the basis of Eagle basic medium. It is one of the most basic and widely used culture medium, and one of the most commonly used culture medium in animal cell culture. MEM medium contains 12 kinds of essential amino acids, glutamine and 8 vitamins, which is simple, mainly used in the culture of adherent cells. It can also be used for other types of cell culture after the formula is modified. ATCC's modified MEM medium contains non-essential amino acids, sodium pyruvate, and reduces the amount of sodium bicarbonate. This product contains many kinds of amino acids, vitamins, inorganic salts and other ingredients for cell culture, but does not contain protein, lipids or any growth factors. Therefore, the product should be used with serum or serum-free additives.

Manufacturer: Elabscience

MEM/F12 medium is based on MEM medium with more abundant nutrients in F12 medium and contains a variety of trace elements. It is widely used in the cultivation of a variety of mammalian cells. At the same time, MEM/F12 medium is often used as the basis for the development of serum-free medium, and it is also suitable for the cultivation of mammalian cells with low serum content. This product contains many kinds of amino acids, vitamins, inorganic salts and other ingredients for cell culture, but does not contain protein, lipids or any growth factors. Therefore, the product should be used with serum or serum-free additives.

Manufacturer: Elabscience

Hep G2 Cell Complete Medium is a ready-to-use medium that can be directly used for the culture of Hep G2 cells, which is carefully designed and optimized by Pricella's R&D team based on MEM, with NEAA basal medium. This high-quality complete medium has been strictly verified by Hep G2 cells which could promote cell proliferation and maintain cell properties.

Manufacturer: Elabscience

Penicillin-streptomycin solution mixture is the most commonly used antibiotic to prevent microbial contamination in vitro. Penicillin can interfere with the synthesis of bacterial cell wall, especially for Gram-positive bacteria. Streptomycin could bind to ribosomal ribosome for 30S and inhibit the synthesis of bacterial protein. It was effective for Gram-negative bacteria and Gram-positive bacteria, but especially for Gram-negative bacteria. Amphotericin B can bind with ergosterol on the membrane of fungi, leading to damage of the membrane, improvement of permeability, leakage of substances inside the cell, disruption of normal metabolism and bacteriostatic effect, but no effect on bacteria. The combined use of penicillin, streptomycin amphotericin B can prevent most bacterial and fungus contamination. But penicillin solution is sensitive to temperature and pH, easy to degrade at room temperature, and needs cryopreservation, the stability of penicillin solution is the most stable when pH is 6.0-6.5. Streptomycin was relatively stable, and pH 5.0-7.5 was the most stable. Amphotericin B solution is unstable at room temperature, easily destroyed by light, heat and acid, and has the strongest antibacterial effect at pH 6.0-7.5.

Manufacturer: Elabscience

NK-92 Cell Complete Medium is a ready-to-use medium that can be directly used for the culture of NK-92 cells, which is carefully designed and optimized by Pricella's R&D team based on MEMα basal medium. This high-quality complete medium has been strictly verified by NK-92 cells which could promote cell proliferation and maintain cell properties.

Manufacturer: Elabscience

DMEM (Dulbecco's Modified Eagle Medium) was developed on the basis of MEM medium. Compared with MEM medium, the content of amino acid increased by 2 times, the content of vitamin increased by 4 times, and the content of non-essential amino acid, trace iron ion and sodium pyruvate were increased by 4 times. The glucose content of DMEM medium was originally designed as 1000 mg/L (low Glucose type), and then developed into 4500 mg/L (high Glucose type), which has been widely used in cell culture. DMEM (High glucose) was widely used in fast growth, low adhesion cells, hybridoma myeloma cells, clone cells, DNA transfected transformation cells, various primary virus host cells, single cell culture and vaccine production. DMEM (High glucose) contains many kinds of amino acids, vitamins, inorganic salts and other ingredients for cell culture, but does not contain protein, lipids or any growth factors, so the product should be used with serum or serum-free additives.