Cell Culture

Manufacturer: Elabscience

Balanced Salt Solution (Physiological Solution) have the properties of buffer solution (regulate pH), normal saline (maintain osmotic pressure) and culture medium (provide nutrition). It can meet the basic needs of survival and metabolism of tissues, organs or cells in vitro. A small amount of phenolic red was added to some equilibrium salt solutions to indicate the pH change of the solution. Dulbecco's Phosphate Buffered Saline (DPBS) is one of the most widely used equilibrium salt solutions in biochemistry. The main components are NaCl, KCl, KH2PO4 and Na2HPO4. DPBS can be divided into two types according to whether contain calcium and magnesium. Different from conventional PBS, DPBS phosphate content was slightly lower. DPBS was mainly used for embryological research.

Manufacturer: Elabscience

Balanced Salt Solution (Physiological Solution) have the properties of buffer solution (regulate pH), normal saline (maintain osmotic pressure) and culture medium (provide nutrition). It can meet the basic needs of survival and metabolism of tissues, organs or cells in vitro. A small amount of phenolic red was added to some equilibrium salt solutions to indicate the pH change of the solution. Hank's Balanced Salt Solution (HBSS) is one of the commonly used phosphate buffers in cell separation or culture. The main components are NaCl, KCl, KH2PO4, Na2HPO4, NaHCO3, CaCl2, MgCl2, MgSO4 and glucose. HBSS is often used to rinse tissue or cells, to transport cells or tissues, to prepare other reagents, to dilute the cells when counting the cells.

Manufacturer: Elabscience

HK-2 Cell Complete Medium is a ready-to-use medium that can be directly used for the culture of HK-2 cells, which is carefully designed and optimized by Pricella's R&D team based on MEM, with NEAA basal medium. This high-quality complete medium has been strictly verified by HK-2 cells which could promote cell proliferation and maintain cell properties.

Manufacturer: Elabscience

Insulin (INS) is a double-stranded (α, β) polypeptide hormone secreted by islet beta cells. It is the only known hormone in the body that reduces blood sugar and simultaneously promotes glycogen, fat, and protein synthesis. Transferrin is an indispensable component in body fluids. It is not only involved in iron transport and metabolism, respiration, cell proliferation and immune system regulation, but also can regulate iron ion balance and energy balance. And it has antibacterial and bactericidal function. Sodium Selenite is a cofactor that provides glutathione peroxidase and other proteins and is used as an antioxidant in culture. Originally used in combination with RPMI 1640 and Minimum Essential Medium (MEM), ITS-G solutions are now commonly used in combination with other basal medium to support multiple cell types in the presence of 2-4?S. ITS is formulated with Earle balanced salt solution (EBSS). Pricella offers three different ITS: ITS-G for general use; ITS-A for adherent cell culture; and ITS-X for cell types requiring aminoethanol, such as bronchial epithelial cells.

Manufacturer: Elabscience

MEM medium (Minimum Essential Medium) was developed on the basis of Eagle basic medium. It is one of the most basic and widely used culture medium, and one of the most commonly used culture medium in animal cell culture. MEM medium contains 12 kinds of essential amino acids, glutamine and 8 vitamins, which is simple, mainly used in the culture of adherent cells. MEM, with NEAA medium is added L-alanine, L-glutamic acid, L-asparagine, L-aspartic acid, L-proline, L-serine and glycine on the basis of MEM medium. These 7 kinds of NEAA, can reduce the side effects of producing non-essential amino acids during cell culture and promote cell proliferation and metabolism. This product contains many kinds of amino acids, vitamins, inorganic salts and other ingredients for cell culture, but does not contain protein, lipids or any growth factors. Therefore, the product should be used with serum or serum-free additives.

Manufacturer: Elabscience

MCF 10A Cell Complete Medium is a ready-to-use medium that can be directly used for the culture of MCF 10A cells, which is carefully designed and optimized by Pricella's R&D team based on DMEM/F12 basal medium. This high-quality complete medium has been strictly verified by MCF 10A cells which could promote cell proliferation and maintain cell properties.

Manufacturer: Elabscience

Insulin is a double-stranded (α, β) peptide hormone secreted by islet β cells. Insulin functions of different species (human, cattle, sheep, pig, etc.) are generally the same, and the composition is slightly different. Insulin is currently known the only hormone in the body that lowers blood sugar, and the only hormone that simultaneously promotes glycogen, fat, and protein synthesis. Insulin plays an important role in many cellular activities. It is a commonly used substance in mammalian cell culture. Insulin can accelerate the use of glucose and inhibit the production of glucose, inhibit the decomposition of fat, promote the synthesis and storage of fat, promote the synthesis of protein, prevents the breakdown of proteins, promotes potassium and magnesium ions to pass through the cell membrane into the cell, promotes the synthesis of deoxyribonucleic acid (DNA), ribonucleic acid (RNA) and adenosine triphosphate (ATP). Recombinant human insulin, produced by E.coli expression system by Elabscience, has a molecular weight of about 5800 Da and has the same structure as human insulin of natural origin. It is often used as an auxiliary component in cell culture. The recommended concentration is 1-10 μg/mL.

Manufacturer: Elabscience

SH-SY5Y Cell Complete Medium is a ready-to-use medium that can be directly used for the culture of SH-SY5Y cells, which is carefully designed and optimized by Pricella's R&D team based on MEM/F12 basal medium. This high-quality complete medium has been strictly verified by SH-SY5Y cells which could promote cell proliferation and maintain cell properties.

Manufacturer: Elabscience

BV2 Cell Complete Medium is a ready-to-use medium that can be directly used for the culture of BV2 cells, which is carefully designed and optimized by Pricella's R&D team based on DMEM basal medium. This high-quality complete medium has been strictly verified by BV2 cells which could promote cell proliferation and maintain cell properties.