RAW 264.7 Cell Complete Medium

Manufacturer: GeneDireX Inc

ITS-M is a mixture of insulin, transferrin, and sodium selenite. It is a general cell supplement designed for use in basal media (e.g. MEM, DMEM, and RPMI-1640) and complex media (e.g. Ham’s F-12 or DMEM/F12).

Manufacturer: GeneDireX Inc

The GeneDireX Ultra293™ is specifically optimized to provide exceptional transfection efficiency of plasmid DNA in HEK 293 and associated cell lineages.

Manufacturer: GeneDireX Inc

The GeneDireX UltraTRAX™ Transfection Reagent is formulated to be a powerful transfection reagent that ensures effective and reproducible transfection with less cytotoxicity.

Manufacturer: GeneDireX Inc

SR DMEM/F-12 (1:1) medium is a new formula basal medium for mammalian cell culture, exhibits low requirement of serum consumption but remains similar constancy of conventional DMEM/F-12 (1:1) medium.

Manufacturer: GeneDireX Inc

SR Buffer is a mixture of nutrients and designed to reduce serum in culture medium for cell proliferation. It is suitable for general basal media (e.g. MEM, DMEM, and RPMI-1640) and complex media (e.g. Ham’s F-12 or DMEM/F12). The usage of SR Buffer is convenient and easy.

Manufacturer: GeneDireX Inc

HybriGO™ SF Medium is a serum-free medium optimized for the growth of a variety of hybridomas and the production of monoclonal antibodies.

Manufacturer: GeneDireX Inc

NeuroGO™ Medium is a basal medium for the special cell culture requirements of pre-natal and embryonic neuronal cells when used with SimplyⓇ B 27 SF Supplement or N2 SF Supplement. NeuroGO™ Medium allows for long-time and short-term maintenance of homogeneous populations of neuronal cells without the need of an astrocyte feeder layer.

Manufacturer: GeneDireX Inc

InsectGO™ SF9/SF21 Medium is a serum-free insect cell culture medium.

Manufacturer: GeneDireX Inc

The serum-free medium optimized for the growth and maintenance of Sf9 and Sf21 cells and for large-scale production of recombinant proteins expressed using the baculovirus expression vector system (BEVS).

Manufacturer: GeneDireX Inc

UltraFect carries most advanced lipid nanoparticle technology to provide superior transfection performance with improved application outcomes and reproducible results. It delivers superior transfection efficiency and improved cell viability.

Manufacturer: GeneDireX Inc

One MEDIUM is a new, novel and easy-to-use cell culture basal medium. It is an innovative application of cell culture methods. It simplifies experimental procedures, in the meanwhile, remains cell proliferation and maintains cellular activities. It is designed to replace the basic medium commonly used in cell culture.

Manufacturer: Abbkine Scientific Co

Abbkine Protease Inhibitor Cocktail is an universal protease inhibitor cocktail contains individual components, including AEBSF, Aprotinin, Bestatin, E-64, Leupeptin and Pepstatin A with a broad specificity for cysteine, serine, acid proteases, and aminopeptidases.

Manufacturer: Abbkine Scientific

SuperKine™ Lipo3.0 Efficient Transfection Reagent is a highly efficient new cationic liposome transfection reagent suitable for plasmid DNA and siRNA transfection in various animal cells, with high transfection efficiency for most animal cells.

Manufacturer: Procell System

DMEM (Dulbecco's Modified Eagle Medium) was developed on the basis of MEM medium. Compared with MEM medium, the content of amino acid increased by 2 times, the content of vitamin increased by 4 times, and the content of non-essential amino acid, trace iron ion and sodium pyruvate were increased by 4 times. The glucose content of DMEM medium was originally designed as 1000 mg/L (low Glucose type), and then developed into 4500 mg/L (high Glucose type), which has been widely used in cell culture. DMEM (High glucose) was widely used in fast growth, low adhesion cells, hybridoma myeloma cells, clone cells, DNA transfected transformation cells, various primary virus host cells, single cell culture and vaccine production. DMEM (High glucose) contains many kinds of amino acids, vitamins, inorganic salts and other ingredients for cell culture, but does not contain protein, lipids or any growth factors, so the product should be used with serum or serum-free additives.

Manufacturer: Procell System

Balanced Salt Solution (Physiological Solution) have the properties of buffer solution (regulate pH), normal saline (maintain osmotic pressure) and culture medium (provide nutrition). It can meet the basic needs of survival and metabolism of tissues, organs or cells in vitro. A small amount of phenolic red was added to some equilibrium salt solutions to indicate the pH change of the solution. Phosphate Buffered Saline (PBS) is one of the most widely used equilibrium salt solutions in biochemistry. The main components are Na2HPO4、KH2PO4、NaCl and KCl. PBS buffers are often used to rinse tissue or cells, to transport cells or tissues, to prepare other reagents, to dilute the cells when counting the cells.

Manufacturer: Procell System

RPMI-1640 is an improved McCoy's 5A medium, which uses bicarbonate buffer system. RPMI-1640 medium was originally designed for lymphocyte culture. Nowadays, it has been widely used in the culture of normal cells and cancer cells, especially suspension cells, which is one of the most widely used media. RPMI-1640 medium contains many kinds of amino acids, vitamins, inorganic salts and other ingredients for cell culture, but does not contain protein, lipids or any growth factors. Therefore, the product should be used serum or serum-free additives.

Manufacturer: Procell System

MEM medium (Minimum Essential Medium) was developed on the basis of Eagle basic medium. It is one of the most basic and widely used culture medium, and one of the most commonly used culture medium in animal cell culture. MEM medium contains 12 kinds of essential amino acids, glutamine and 8 vitamins, which is simple, mainly used in the culture of adherent cells. MEM, with NEAA medium is added L-alanine, L-glutamic acid, L-asparagine, L-aspartic acid, L-proline, L-serine and glycine on the basis of MEM medium. These 7 kinds of NEAA, can reduce the side effects of producing non-essential amino acids during cell culture and promote cell proliferation and metabolism. This product contains many kinds of amino acids, vitamins, inorganic salts and other ingredients for cell culture, but does not contain protein, lipids or any growth factors. Therefore, the product should be used with serum or serum-free additives.

Manufacturer: Procell System

The DMEM/F12 medium is based on the DMEM medium, adding more nutritious elements from F12 medium, which contains a variety of trace elements, and is widely used in the culture of many mammalian cells. At the same time, DMEM/F12 medium is often used as the basis for the development of serum-free medium. It is also suitable for mammalian cell culture and clone density culture under low serum content. This product contains many kinds of amino acids, vitamins, inorganic salts and other ingredients for cell culture, but does not contain protein, lipids or any growth factors. Therefore, the product should be used with serum or serum-free additives.

Manufacturer: Procell System

Balanced Salt Solution (Physiological Solution) have the properties of buffer solution (regulate pH), normal saline (maintain osmotic pressure) and culture medium (provide nutrition). It can meet the basic needs of survival and metabolism of tissues, organs or cells in vitro. A small amount of phenolic red was added to some equilibrium salt solutions to indicate the pH change of the solution. Dulbecco's Phosphate Buffered Saline (DPBS) is one of the most widely used equilibrium salt solutions in biochemistry. The main components are NaCl, KCl, KH2PO4 and Na2HPO4. DPBS can be divided into two types according to whether contain calcium and magnesium. Different from conventional PBS, DPBS phosphate content was slightly lower. DPBS was mainly used for embryological research.

Manufacturer: Procell System

McCoy's 5A medium was initially used specifically for the culture of Novikoff Hepatoma cells. Unlike other medium, McCoy's 5A medium contains reduced glutathione, bacterial peptone and high concentrations of glucose. McCoy's 5A is widely used in the culture of primary cells, such as bone marrow, skin, gingiva, kidney, spleen, lung, rat embryo, omentum and so on. In addition, McCoy's 5A medium is also used for tissue biopsy culture, the establishment of cell line, and the culture of some lymphocytes and more difficult cells. This product contains many kinds of amino acids, vitamins, inorganic salts and other ingredients for cell culture, but does not contain protein, lipids or any growth factors. Therefore, the product should be used with serum or serum-free additives.

Manufacturer: Procell System

Trypsin is a serine hydrolase that can cut the base-side segment of the lysine and arginine residue in the polypeptide chain, hydrolyze the protein between the cells, and destroy the connection between the cells, so that the tissue or the adherent cells are dispersed into a single cell. The activity of the trypsin is related to the characteristics of the tissue or the cells, the concentration of the trypsin, the temperature and the time of action. At pH 8.0 and 37℃, the effect of the trypsin is the best. Therefore, the concentration of trypsin, the temperature and the time of the pancreatic enzyme should be grasped to avoid the excessive damage of the cells. In general, the frequently-used working concentration of the trypsin is 0.25%, while the concentration of trypsin for the semi-adherent cells or the cells is 0.05%. Since EDTA is able to bind Ca2+ and Mg2+ to destroy the cell connection to promote the dissociation of the cells, a certain amount of EDTA is often added to the trypsin solution to enhance the dissociation effect.

Manufacturer: Procell System

Penicillin-streptomycin solution mixture is the most commonly used antibiotic to prevent microbial contamination in vitro. Penicillin can interfere with the synthesis of bacterial cell wall, especially for Gram-positive bacteria. Streptomycin could bind to ribosomal ribosome for 30S and inhibit the synthesis of bacterial protein. It was effective for Gram-negative bacteria and Gram-positive bacteria, but especially for Gram-negative bacteria. The combined use of penicillin and streptomycin can prevent most bacterial contamination, but penicillin solution is sensitive to temperature and pH, easy to degrade at room temperature, and needs cryopreservation, the stability of penicillin solution is the most stable when pH is 6.0-6.5. Streptomycin was relatively stable, and pH 5.0-7.5 was the most stable.

Manufacturer: Procell System

RAW 264.7 Cell Complete Medium is a ready-to-use medium that can be directly used for the culture of RAW 264.7 cells, which is carefully designed and optimized by Pricella's R&D team based on DMEM basal medium. This high-quality complete medium has been strictly verified by RAW 264.7 cells which could promote cell proliferation and maintain cell properties.

Manufacturer: Procell System

The Ham's F-12K medium increases the concentration of amino acids and pyruvic acid on the basis of Ham's F-12, reduces the concentration of glucose, and modifies the composition and content of the salt.The Ham's F-12K medium was initially designed to culture primary human hepatocytes and differentiated cells of rats and chickens. The Ham's F-12K medium contains many kinds of amino acids, vitamins, inorganic salts and other ingredients for cell culture, but does not contain protein, lipids or any growth factors. Therefore, the product should be used with serum or serum-free additives.

Manufacturer: Procell System

In vitro cell culture, in order to preserve the biological activity of cells for a long time, the cells must be cryopreserved, and resuscitated when necessary. At present, the most commonly used method for cell cryopreservation is liquid nitrogen method. In the process, add an appropriate amount of protective agent to slowly cool the cells to the specified temperature range, so as to achieve the purpose of protecting the cells. The Serum-free Cell Freezing Medium is a special cryopreservation product developed by Pricella for hundreds of types of cells by continuously optimizing experimental conditions for cell cryopreservation and resuscitation in the long-term cell research. The product adds cell sedimentation stabilizer, which can delay the sedimentation rate of cells in the process of cryopreservation, prevent cells from squeezing each other and affect the effect of cell cryopreservation. In addition, a variety of cryoprotectants, such as cell membrane protectors, permeable intracellular membrane protectors, and non-permeable cell protectors, are added. These components combine with water molecules in the solution to hydrate and weaken the crystallization process of water, increasing the viscosity of the solution and reducing the formation of ice crystals, which can greatly reduce the damage of ice crystals to cells during cryopreservation, and effectively improve the survival rate of cell after resuscitation. The product formula is clear, does not contain serum or animal derived protein, which can reduce comtamination risk from bacteria, viruses and mycoplasma, and ensure the safety of frozen cells. It is not only suitable for conventional cell lines, primary cells, but also for serum-free culture cells and protein-expressing cells. Compared with traditional cryopreservation media, there is no need for tedious and time-consuming gradient freezing steps, and no need for expensive program cooling equipment. Cells can be directly resuspended and placed at -80℃, and transferred to liquid nitrogen the next day to complete the entire process, saving a lot of time and energy.

Manufacturer: Procell System

MEMα was developed on the basis of MEM medium. Compared with MEM medium, NEAA, sodium pyruvate, zinc sulfate, VB12, biotin and ascorbic acid were added. It is widely used in suspension and adherent cell culture of various mammals. MEMα medium without nucleoside and deoxynucleoside is often used as medium for DG44 and other DHFR- cells. This product contains many kinds of amino acids, vitamins, inorganic salts and other ingredients for cell culture, but does not contain protein, lipids or any growth factors. Therefore, the product should be used with serum or serum-free additives.

Manufacturer: Procell System

Trypsin is a serine hydrolase that can cut the base-side segment of the lysine and arginine residue in the polypeptide chain, hydrolyze the protein between the cells, and destroy the connection between the cells, so that the tissue or the adherent cells are dispersed into a single cell. The activity of the trypsin is related to the characteristics of the tissue or the cells, the concentration of the trypsin, the temperature and the time of action. At pH 8.0 and 37℃, the effect of the trypsin is the best. Therefore, the concentration of trypsin, the temperature and the time of the pancreatic enzyme should be grasped to avoid the excessive damage of the cells. In general, the frequently-used working concentration of the trypsin is 0.25%, while the concentration of trypsin for the semi-adherent cells or the cells is 0.05%. Since EDTA is able to bind Ca2+ and Mg2+ to destroy the cell connection to promote the dissociation of the cells, a certain amount of EDTA is often added to the trypsin solution to enhance the dissociation effect.

Manufacturer: Procell System

DMEM (Dulbecco's Modified Eagle Medium) was developed on the basis of MEM medium. Compared with MEM medium, the content of amino acid increased by 2 times, the content of vitamin increased by 4 times, and the content of non-essential amino acid, trace iron ion and sodium pyruvate were increased by 4 times. The glucose content of DMEM medium was originally designed as 1000 mg/L (low Glucose type), and then developed into 4500 mg/L (high Glucose type), which has been widely used in cell culture. DMEM (High glucose) was widely used in fast growth, low adhesion cells, hybridoma myeloma cells, clone cells, DNA transfected transformation cells, various primary virus host cells, single cell culture and vaccine production. DMEM (High glucose) contains many kinds of amino acids, vitamins, inorganic salts and other ingredients for cell culture, but does not contain protein, lipids or any growth factors, so the product should be used with serum or serum-free additives.

Manufacturer: Procell System

The DMEM/F12 medium is based on the DMEM medium, adding more nutritious elements from F12 medium, which contains a variety of trace elements, and is widely used in the culture of many mammalian cells. At the same time, DMEM/F12 medium is often used as the basis for the development of serum-free medium. It is also suitable for mammalian cell culture and clone density culture under low serum content. This product contains many kinds of amino acids, vitamins, inorganic salts and other ingredients for cell culture, but does not contain protein, lipids or any growth factors. Therefore, the product should be used with serum or serum-free additives.

Manufacturer: Procell System

Nanobacteria and their decomposition complexes are the common contaminant in cell cultures that co-exists with cells. Antibiotics are usually ineffective. Nanobacteria grows competitively with cells, which is unfavorable to cell growth, and in severe cases causes cell death. At present, many cells are contaminated with nanobacteria, which has a great impact on cell culture and subsequent experiments. The common characteristics of cells contaminated by nanobacteria are as follows: (1) The medium is not turbid, but when the cells are observed under a microscope, there are many "small black spots" around the cells or in the culture medium. With the extension of culture time, the "small black spots" gradually increase, and they cannot be removed by changing the culture medium or washing the cells (2) The cells contaminated by the "small black spots" consume fast nutrients and require frequent replacement of the culture medium (3) Nanobacteria-contaminated cells grow slowly, have poor cell states, and are severely vacuolated. They may even cause changes in cell morphology. Therefore, it is of great significance to clean up nanobacteria contamination in cell culture.

Manufacturer: Procell System

DMEM (Dulbecco's Modified Eagle Medium) was developed on the basis of MEM medium. Compared with MEM medium, the content of amino acid increased by 2 times, the content of vitamin increased by 4 times, and the content of non-essential amino acid, trace iron ion and sodium pyruvate were increased by 4 times. The glucose content of DMEM medium was originally designed as 1000 mg/L (low Glucose type), and then developed into 4500 mg/L (high Glucoser type), which has been widely used in cell culture. DMEM (Low glucose) contains many kinds of amino acids, vitamins, inorganic salts and other ingredients for cell culture, but does not contain protein, lipids or any growth factors, so the product should be used with serum or serum-free additives.

Manufacturer: Procell System

RPMI-1640 is an improved Mccoy's 5A medium, which uses bicarbonate buffer system. RPMI-1640 medium was originally designed for lymphocyte culture. Nowadays, it has been widely used in the culture of normal cells and cancer cells, especially suspension cells, which is one of the most widely used media. RPMI-1640 medium contains many kinds of amino acids, vitamins, inorganic salts and other ingredients for cell culture, but does not contain protein, lipids or any growth factors. Therefore, the product should be used serum or serum-free additives.

Manufacturer: Procell System

Mycoplasma is the smallest and simplest prokaryote found so far, with a diameter of 0.1-0.3 μm. It can easily pass through the filter membrane and enter the cell culture system. It has various forms such as sphere, rod, filamentous and branching. Usually attached to the surface of the cell membrane, there is no cell wall, so ordinary antibiotics have no effect on it. Mycoplasma contamination is widespread and often underestimated in cell cultures. Mycoplasma contamination can cause many problems, such as changes in cell growth rate, cell morphology, chromosome aberrations, cell membrane antigenicity changes, cell metabolism changes, reduced survival rate after cell resuscitation, and so on, which ultimately leads to inaccurate or incorrect experimental results. Therefore, it is of great significance to clean up mycoplasma contamination in cell culture.

Manufacturer: Procell System

THP-1 Cell Complete Medium is a ready-to-use medium that can be directly used for the culture of THP-1 cells, which is carefully designed and optimized by Pricella's R&D team based on RPMI-1640 basal medium. This high-quality complete medium has been strictly verified by THP-1 cells which could promote cell proliferation and maintain cell properties.

Manufacturer: Procell System

IMDM (Iscove's Modified Dulbecco Medium) is an improved DMEM medium, which is used for the culture of erythrocyte progenitor cells and macrophages. The IMDM medium was supplemented with selenium, HEPES, sodium pyruvate and additional amino acids and vitamins on the basis of the DMEM medium, and potassium nitrate instead of iron nitrate, which is suitable for the rapid proliferation, high-density cell culture. IMDM medium can not only culture cells with special nutritional requirements (such as mouse B lymphocytes, stimulated B cells stimulated by LPS, bone marrow hematopoietic cells, T lymphocytes and various hybridoma cells), It can also be used as a base solution for some unique serum-free medium. This product contains many kinds of amino acids, vitamins, inorganic salts and other ingredients for cell culture, but does not contain protein, lipids or any growth factors. Therefore, the product should be used with serum or serum-free additives.

Manufacturer: Procell System

DMEM (Dulbecco's Modified Eagle Medium) was developed on the basis of MEM medium. Compared with MEM medium, the content of amino acid increased by 2 times, the content of vitamin increased by 4 times, and the content of non-essential amino acid, trace iron ion and sodium pyruvate were increased by 4 times. The glucose content of DMEM medium was originally designed as 1000 mg/L (low Glucose type), and then developed into 4500 mg/L (high Glucoser type), which has been widely used in cell culture. DMEM medium without glucose can be added to the concentration of glucose at will according to the research needs, which is convenient and quick. DMEM (glucose free) contains many kinds of amino acids, vitamins, inorganic salts and other ingredients for cell culture, but does not contain protein, lipids or any growth factors, so the product should be used with serum or serum-free additives.

Manufacturer: Procell System

Balanced Salt Solution (Physiological Solution) have the properties of buffer solution (regulate pH), normal saline (maintain osmotic pressure) and culture medium (provide nutrition). It can meet the basic needs of survival and metabolism of tissues, organs or cells in vitro. A small amount of phenolic red was added to some equilibrium salt solutions to indicate the pH change of the solution. Hank's Balanced Salt Solution (HBSS) is one of the commonly used phosphate buffers in cell separation or culture. The main components are NaCl、KCl、KH2PO4、Na2HPO4、NaHCO3 and glucose. HBSS is used for the washing of cells before passage and tissue dissociation, preparation of cell dissociation reagent, transport of cell or tissue, dilution of cells, etc.

Manufacturer: Procell System

Trypsin is a serine hydrolase that can cut the base-side segment of the lysine and arginine residue in the polypeptide chain, hydrolyze the protein between the cells, and destroy the connection between the cells, so that the tissue or the adherent cells are dispersed into a single cell. The activity of the trypsin is related to the characteristics of the tissue or the cells, the concentration of the trypsin, the temperature and the time of action. At pH 8.0 and 37℃, the effect of the trypsin is the best. Therefore, the concentration of trypsin, the temperature and the time of the pancreatic enzyme should be grasped to avoid the excessive damage of the cells. In general, the frequently-used working concentration of the trypsin is 0.25%, while the concentration of trypsin for the semi-adherent cells or the cells is 0.05%. Since EDTA is able to bind Ca2+ and Mg2+ to destroy the cell connection to promote the dissociation of the cells, a certain amount of EDTA is often added to the trypsin solution to enhance the dissociation effect.

Manufacturer: Procell System

Ham's F-12 Nutrient Mixture was designed by Ham in 1969 based on Ham's F-10 nutrient mixture, which was originally used for serum-free culture of CHO cells. Ham's F-12 is often used as the basic culture medium for serum free culture. At low serum levels, it is especially suitable for single cell culture and clonal culture. After adding serum, it is also widely used in the culture of cancer cells and primary cells, such as rat hepatocytes, rat prostate epithelial cells, chondrocytes, rat myoblasts, and chicken embryonic cells. In addition, the DMEM/F12 medium obtained by mixing Ham's F-12 in equal volume with DMEM is more nutritious and widely used.

Manufacturer: Procell System

The buffer system of Leibovitz's L-15 medium is composed of phosphate and free amino acids, instead of the traditional sodium bicarbonate buffer system. Meanwhile, the substitution of galactose and sodium pyruvate for glucose prevents prevents the formation of acidic metabolic by-products, helps to maintain the stable pH of the medium. It is suitable for cell culture in a non-CO2 balanced environment. Leibovitz's L-15 medium is suitable for the culture of monkey kidney cells and HEP-2, the isolation of primary cells (such as embryonic and adult tissues), the culture of various viruses and the culture of neurons. Leibovitz's L-15 medium contains many kinds of amino acids, vitamins, inorganic salts and other ingredients for cell culture, but does not contain protein, lipids or any growth factors. Therefore, the product should be used with serum or serum-free additives.

Manufacturer: Procell System

AML12 Cell Complete Medium is a ready-to-use medium that can be directly used for the culture of AML12 cells, which is carefully designed and optimized by Pricella's R&D team based on DMEM/F12 basal medium. This high-quality complete medium has been strictly verified by AML12 cells which could promote cell proliferation and maintain cell properties.

Manufacturer: Procell System

BV2 Cell Complete Medium is a ready-to-use medium that can be directly used for the culture of BV2 cells, which is carefully designed and optimized by Pricella's R&D team based on DMEM basal medium. This high-quality complete medium has been strictly verified by BV2 cells which could promote cell proliferation and maintain cell properties.

Manufacturer: Procell System

SH-SY5Y Cell Complete Medium is a ready-to-use medium that can be directly used for the culture of SH-SY5Y cells, which is carefully designed and optimized by Pricella's R&D team based on MEM/F12 basal medium. This high-quality complete medium has been strictly verified by SH-SY5Y cells which could promote cell proliferation and maintain cell properties.

Manufacturer: Procell System

Insulin is a double-stranded (α, β) peptide hormone secreted by islet β cells. Insulin functions of different species (human, cattle, sheep, pig, etc.) are generally the same, and the composition is slightly different. Insulin is currently known the only hormone in the body that lowers blood sugar, and the only hormone that simultaneously promotes glycogen, fat, and protein synthesis. Insulin plays an important role in many cellular activities. It is a commonly used substance in mammalian cell culture. Insulin can accelerate the use of glucose and inhibit the production of glucose, inhibit the decomposition of fat, promote the synthesis and storage of fat, promote the synthesis of protein, prevents the breakdown of proteins, promotes potassium and magnesium ions to pass through the cell membrane into the cell, promotes the synthesis of deoxyribonucleic acid (DNA), ribonucleic acid (RNA) and adenosine triphosphate (ATP). Recombinant human insulin, produced by E.coli expression system by Elabscience, has a molecular weight of about 5800 Da and has the same structure as human insulin of natural origin. It is often used as an auxiliary component in cell culture. The recommended concentration is 1-10 μg/mL.

Manufacturer: Procell System

MCF 10A Cell Complete Medium is a ready-to-use medium that can be directly used for the culture of MCF 10A cells, which is carefully designed and optimized by Pricella's R&D team based on DMEM/F12 basal medium. This high-quality complete medium has been strictly verified by MCF 10A cells which could promote cell proliferation and maintain cell properties.

Manufacturer: Procell System

MEM medium (Minimum Essential Medium) was developed on the basis of Eagle basic medium. It is one of the most basic and widely used culture medium, and one of the most commonly used culture medium in animal cell culture. MEM medium contains 12 kinds of essential amino acids, glutamine and 8 vitamins, which is simple, mainly used in the culture of adherent cells. MEM, with NEAA medium is added L-alanine, L-glutamic acid, L-asparagine, L-aspartic acid, L-proline, L-serine and glycine on the basis of MEM medium. These 7 kinds of NEAA, can reduce the side effects of producing non-essential amino acids during cell culture and promote cell proliferation and metabolism. This product contains many kinds of amino acids, vitamins, inorganic salts and other ingredients for cell culture, but does not contain protein, lipids or any growth factors. Therefore, the product should be used with serum or serum-free additives.

Manufacturer: Procell System

Insulin (INS) is a double-stranded (α, β) polypeptide hormone secreted by islet beta cells. It is the only known hormone in the body that reduces blood sugar and simultaneously promotes glycogen, fat, and protein synthesis. Transferrin is an indispensable component in body fluids. It is not only involved in iron transport and metabolism, respiration, cell proliferation and immune system regulation, but also can regulate iron ion balance and energy balance. And it has antibacterial and bactericidal function. Sodium Selenite is a cofactor that provides glutathione peroxidase and other proteins and is used as an antioxidant in culture. Originally used in combination with RPMI 1640 and Minimum Essential Medium (MEM), ITS-G solutions are now commonly used in combination with other basal medium to support multiple cell types in the presence of 2-4?S. ITS is formulated with Earle balanced salt solution (EBSS). Pricella offers three different ITS: ITS-G for general use; ITS-A for adherent cell culture; and ITS-X for cell types requiring aminoethanol, such as bronchial epithelial cells.

Manufacturer: Procell System

HK-2 Cell Complete Medium is a ready-to-use medium that can be directly used for the culture of HK-2 cells, which is carefully designed and optimized by Pricella's R&D team based on MEM, with NEAA basal medium. This high-quality complete medium has been strictly verified by HK-2 cells which could promote cell proliferation and maintain cell properties.

Manufacturer: Procell System

Balanced Salt Solution (Physiological Solution) have the properties of buffer solution (regulate pH), normal saline (maintain osmotic pressure) and culture medium (provide nutrition). It can meet the basic needs of survival and metabolism of tissues, organs or cells in vitro. A small amount of phenolic red was added to some equilibrium salt solutions to indicate the pH change of the solution. Hank's Balanced Salt Solution (HBSS) is one of the commonly used phosphate buffers in cell separation or culture. The main components are NaCl, KCl, KH2PO4, Na2HPO4, NaHCO3, CaCl2, MgCl2, MgSO4 and glucose. HBSS is often used to rinse tissue or cells, to transport cells or tissues, to prepare other reagents, to dilute the cells when counting the cells.

Manufacturer: Procell System

Balanced Salt Solution (Physiological Solution) have the properties of buffer solution (regulate pH), normal saline (maintain osmotic pressure) and culture medium (provide nutrition). It can meet the basic needs of survival and metabolism of tissues, organs or cells in vitro. A small amount of phenolic red was added to some equilibrium salt solutions to indicate the pH change of the solution. Dulbecco's Phosphate Buffered Saline (DPBS) is one of the most widely used equilibrium salt solutions in biochemistry. The main components are NaCl, KCl, KH2PO4 and Na2HPO4. DPBS can be divided into two types according to whether contain calcium and magnesium. Different from conventional PBS, DPBS phosphate content was slightly lower. DPBS was mainly used for embryological research.

Manufacturer: Procell System

DMEM (Dulbecco's Modified Eagle Medium) was developed on the basis of MEM medium. Compared with MEM medium, the content of amino acid increased by 2 times, the content of vitamin increased by 4 times, and the content of non-essential amino acid, trace iron ion and sodium pyruvate were increased by 4 times. The glucose content of DMEM medium was originally designed as 1000 mg/L (low Glucose type), and then developed into 4500 mg/L (high Glucose type), which has been widely used in cell culture. DMEM (High glucose) was widely used in fast growth, low adhesion cells, hybridoma myeloma cells, clone cells, DNA transfected transformation cells, various primary virus host cells, single cell culture and vaccine production. DMEM (High glucose) contains many kinds of amino acids, vitamins, inorganic salts and other ingredients for cell culture, but does not contain protein, lipids or any growth factors, so the product should be used with serum or serum-free additives.

Manufacturer: Procell System

NK-92 Cell Complete Medium is a ready-to-use medium that can be directly used for the culture of NK-92 cells, which is carefully designed and optimized by Pricella's R&D team based on MEMα basal medium. This high-quality complete medium has been strictly verified by NK-92 cells which could promote cell proliferation and maintain cell properties.

Manufacturer: Procell System

Penicillin-streptomycin solution mixture is the most commonly used antibiotic to prevent microbial contamination in vitro. Penicillin can interfere with the synthesis of bacterial cell wall, especially for Gram-positive bacteria. Streptomycin could bind to ribosomal ribosome for 30S and inhibit the synthesis of bacterial protein. It was effective for Gram-negative bacteria and Gram-positive bacteria, but especially for Gram-negative bacteria. Amphotericin B can bind with ergosterol on the membrane of fungi, leading to damage of the membrane, improvement of permeability, leakage of substances inside the cell, disruption of normal metabolism and bacteriostatic effect, but no effect on bacteria. The combined use of penicillin, streptomycin amphotericin B can prevent most bacterial and fungus contamination. But penicillin solution is sensitive to temperature and pH, easy to degrade at room temperature, and needs cryopreservation, the stability of penicillin solution is the most stable when pH is 6.0-6.5. Streptomycin was relatively stable, and pH 5.0-7.5 was the most stable. Amphotericin B solution is unstable at room temperature, easily destroyed by light, heat and acid, and has the strongest antibacterial effect at pH 6.0-7.5.

Manufacturer: Procell System

Hep G2 Cell Complete Medium is a ready-to-use medium that can be directly used for the culture of Hep G2 cells, which is carefully designed and optimized by Pricella's R&D team based on MEM, with NEAA basal medium. This high-quality complete medium has been strictly verified by Hep G2 cells which could promote cell proliferation and maintain cell properties.

Manufacturer: Procell System

MEM/F12 medium is based on MEM medium with more abundant nutrients in F12 medium and contains a variety of trace elements. It is widely used in the cultivation of a variety of mammalian cells. At the same time, MEM/F12 medium is often used as the basis for the development of serum-free medium, and it is also suitable for the cultivation of mammalian cells with low serum content. This product contains many kinds of amino acids, vitamins, inorganic salts and other ingredients for cell culture, but does not contain protein, lipids or any growth factors. Therefore, the product should be used with serum or serum-free additives.

Manufacturer: Procell System

MEM medium (Minimum Essential Medium) was developed on the basis of Eagle basic medium. It is one of the most basic and widely used culture medium, and one of the most commonly used culture medium in animal cell culture. MEM medium contains 12 kinds of essential amino acids, glutamine and 8 vitamins, which is simple, mainly used in the culture of adherent cells. It can also be used for other types of cell culture after the formula is modified. ATCC's modified MEM medium contains non-essential amino acids, sodium pyruvate, and reduces the amount of sodium bicarbonate. This product contains many kinds of amino acids, vitamins, inorganic salts and other ingredients for cell culture, but does not contain protein, lipids or any growth factors. Therefore, the product should be used with serum or serum-free additives.

Manufacturer: Procell System

M199 (Medium 199) originally used in nutritional studies of chicken embryo fibroblasts, it has been widely used in the culture of various animal cells, including some non-mammalian cells. M199 is especially suitable for the culture of untransformed cells, rat pancreatic epithelial cells and mouse lens tissues, also used in virology and vaccine production. Compared with other basic medium, M199 contains unique ingredients, including adenine, adenosine, hypoxanthine, thymine, and other vitamins. There are two kinds of equilibrium salt (earle's salt and hank's salt) in M199. Earle's salt is often used in CO2 environment, hank's salt is used in non-CO2 environment. This product contains many kinds of amino acids, vitamins, inorganic salts and other ingredients for cell culture, but does not contain protein, lipids or any growth factors. Therefore, the product should be used with serum or serum-free additives.

Manufacturer: Procell System

L-glutamine is an essential element in cell culture that is not only an energy source of the cells, but also an important substance involved in protein synthesis and nucleic acid metabolism. The deficiency of L-glutamine will lead to poor cell growth and even death. L-glutamine is unstable in solution and easy to degrade spontaneously, so it is necessary to supplement a certain amount of L-glutamine in culture medium to meet the needs of cell growth after a period of time or when cells grow badly.

Manufacturer: Procell System

Caco-2 Cell Complete Medium is a ready-to-use medium that can be directly used for the culture of Caco-2 cells, which is carefully designed and optimized by Pricella's R&D team based on MEM, with NEAA basal medium. This high-quality complete medium has been strictly verified by Caco-2 cells which could promote cell proliferation and maintain cell properties.

Manufacturer: Procell System

293T [HEK-293T] Cell Complete Medium is a ready-to-use medium that can be directly used for the culture of 293T [HEK-293T] cells, which is carefully designed and optimized by Pricella's R&D team based on DMEM basal medium. This high-quality complete medium has been strictly verified by 293T [HEK-293T] cells which could promote cell proliferation and maintain cell properties.

Manufacturer: Procell System

4T1 Cell Complete Medium is a ready-to-use medium that can be directly used for the culture of 4T1 cells, which is carefully designed and optimized by Pricella's R&D team based on RPMI-1640 basal medium. This high-quality complete medium has been strictly verified by 4T1 cells which could promote cell proliferation and maintain cell properties.

Manufacturer: Procell System

Amino Acids are the basic unit of protein composition and also an important component of life. According to the cell's own ability to synthesize, amino acids can be divided into three categories: essential amino acids, semi-essential amino acids, and non-essential amino acids. MEM non-essential amino acid solution (NEAA) contain L-alanine, L-glutamic acid, L-asparaginate, L-proline, L-aspartic acid, L-serine and glycine, can effectively improve the ratio of cells culture medium, reduce the side effect of non-essential amino acid production in cell culture, and promote cell proliferation and metabolism, which is one of the commonly used additives in cell culture.

Manufacturer: Procell System

A549 [A-549] Cell Complete Medium is a ready-to-use medium that can be directly used for the culture of A549 [A-549] cells, which is carefully designed and optimized by Pricella's R&D team based on Ham's F-12K basal medium. This high-quality complete medium has been strictly verified by A549 [A-549] cells which could promote cell proliferation and maintain cell properties.

Manufacturer: Procell System

Poly-L-Lysine (PLL) is a Poly-cation polymerized from lysine monomer.With a relative molecular weight of 70,000-300,000, PLL can be used as the growth matrix of cell culture, which can promote cell adhesion and adhesion to the wall. PLL is a commonly used cell adhesion agent in cell culture. Polyl-l-lysine (PLL) generally has molecular weight of 70,000-150,000, 150,000-300,000 and > 300,000. The larger the molecular weight, the stronger the adhesion, but the more difficult it is to completely dissolve. Polyl-l-lysine with molecular weight of 150,000-300,000 is commonly used in cell culture.

Manufacturer: Procell System

RPMI-1640 is an improved McCoy's 5A medium, which uses bicarbonate buffer system. RPMI-1640 medium was originally designed for lymphocyte culture. Nowadays, it has been widely used in the culture of normal cells and cancer cells, especially suspension cells, which is one of the most widely used media. RPMI-1640 medium contains many kinds of amino acids, vitamins, inorganic salts and other ingredients for cell culture, but does not contain protein, lipids or any growth factors. Therefore, the product should be used serum or serum-free additives.

Manufacturer: Procell System

THLE-2 Cell Complete Medium is a ready-to-use medium that can be directly used for the culture of THLE-2 cells, which is carefully designed and optimized by Pricella's R&D team. This high-quality complete medium has been strictly verified by THLE-2 cells which could promote cell proliferation and maintain cell properties

Manufacturer: Procell System

DMEM (Dulbecco's Modified Eagle Medium) was developed on the basis of MEM medium. Compared with MEM medium, the content of amino acid increased by 2 times, the content of vitamin increased by 4 times, and the content of non-essential amino acid, trace iron ion and sodium pyruvate were increased by 4 times. The glucose content of DMEM medium was originally designed as 1000 mg/L (low Glucose type), and then developed into 4500 mg/L (high Glucose type), which has been widely used in cell culture. DMEM (High glucose) was widely used in fast growth, low adhesion cells, hybridoma myeloma cells, clone cells, DNA transfected transformation cells, various primary virus host cells, single cell culture and vaccine production. DMEM (High glucose) contains many kinds of amino acids, vitamins, inorganic salts and other ingredients for cell culture, but does not contain protein, lipids or any growth factors, so the product should be used with serum or serum-free additives.

Manufacturer: Procell System

RPMI-1640 is an improved McCoy's 5A medium, which uses bicarbonate buffer system. RPMI-1640 medium was originally designed for lymphocyte culture. Nowadays, it has been widely used in the culture of normal cells and cancer cells, especially suspension cells, which is one of the most widely used media. RPMI-1640 medium contains many kinds of amino acids, vitamins, inorganic salts and other ingredients for cell culture, but does not contain protein, lipids or any growth factors. Therefore, the product should be used serum or serum-free additives.

Manufacturer: Procell System

MC3T3-E1 Subclone 14 Cell Complete Medium is a ready-to-use medium that can be directly used for the culture of MC3T3-E1 Subclone 14 cells, which is carefully designed and optimized by Pricella's R&D team based on MEMα basal medium. This high-quality complete medium has been strictly verified by MC3T3-E1 Subclone 14 cells which could promote cell proliferation and maintain cell properties.

Manufacturer: Procell System

Sodium Pyruvate, the most common pyruvate, it is an intermediate product of glycolysis. It can directly enter and exit cells. It is a necessary nutrient for cell metabolism. In the absence of glucose, it can be used as a source of energy for cells. It is one of the commonly used additives in cell culture. Although cells can produce sodium pyruvate, some cells require additional addition of sodium pyruvate during in vitro culture. A certain amount of sodium pyruvate can also be added during clonal culture or low level of serum concentration, and sodium pyruvate also can reduce phototoxic effects caused by fluorescent substances.

Manufacturer: Procell System

H9c2(2-1) Cell Complete Medium is a ready-to-use medium that can be directly used for the culture of H9c2(2-1) cells, which is carefully designed and optimized by Pricella's R&D team based on DMEM basal medium. This high-quality complete medium has been strictly verified by H9c2(2-1) cells which could promote cell proliferation and maintain cell properties.

Manufacturer: Procell System

DMEM (Dulbecco's Modified Eagle Medium) was developed on the basis of MEM medium. Compared with MEM medium, the content of amino acid increased by 2 times, the content of vitamin increased by 4 times, and the content of non-essential amino acid, trace iron ion and sodium pyruvate were increased by 4 times. The glucose content of DMEM medium was originally designed as 1000 mg/L (low Glucose type), and then developed into 4500 mg/L (high Glucose type), which has been widely used in cell culture. DMEM (High glucose) was widely used in fast growth, low adhesion cells, hybridoma myeloma cells, clone cells, DNA transfected transformation cells, various primary virus host cells, single cell culture and vaccine production. DMEM (High glucose) contains many kinds of amino acids, vitamins, inorganic salts and other ingredients for cell culture, but does not contain protein, lipids or any growth factors, so the product should be used with serum or serum-free additives.

Manufacturer: Procell System

HEPES is all known as N-2-hydroxyethylpiperazine-N-2-ethane sulfonic acid, is a commonly used biological buffer. pKa (25℃) is 7.5 and an effective buffer range of pH is 6.8-8.2. The greatest advantage of HEPES is that to maintain the pH range of a solution for a longer period of time in an open environment. HEPES cannot pass through biofilms. It is a chemical and enzymatic stable substance with limited effect on biochemical reactions, and the absorption of visible and ultraviolet light is extremely low. Therefore, it is generally considered that HEPES is an excellent biological buffer, and there is no toxic effect to the cells. The working concentration is 10-50 mM and the final concentration for cell culture is 10 mM.

Manufacturer: Procell System

HT22 Cell Complete Medium is a ready-to-use medium that can be directly used for the culture of HT22 cells, which is carefully designed and optimized by Pricella's R&D team based on DMEM basal medium. This high-quality complete medium has been strictly verified by HT22 cells which could promote cell proliferation and maintain cell properties.

Manufacturer: Procell System

3T3-L1 Cell Complete Medium is a ready-to-use medium that can be directly used for the culture of 3T3-L1 cells, which is carefully designed and optimized by Pricella's R&D team based on DMEM basal medium. This high-quality complete medium has been strictly verified by 3T3-L1 cells which could promote cell proliferation and maintain cell properties.

Manufacturer: Procell System

RPMI-1640 is an improved McCoy's 5A medium, which uses bicarbonate buffer system. RPMI-1640 medium was originally designed for lymphocyte culture. Nowadays, it has been widely used in the culture of normal cells and cancer cells, especially suspension cells, which is one of the most widely used media. RPMI-1640 medium contains many kinds of amino acids, vitamins, inorganic salts and other ingredients for cell culture, but does not contain protein, lipids or any growth factors. Therefore, the product should be used serum or serum-free additives.

Manufacturer: Procell System

MDA-MB-231 Cell Complete Medium is a ready-to-use medium that can be directly used for the culture of MDA-MB-231 cells, which is carefully designed and optimized by Pricella's R&D team based on DMEM basal medium. This high-quality complete medium has been strictly verified by MDA-MB-231 cells which could promote cell proliferation and maintain cell properties.

Manufacturer: Procell System

MCF7 [MCF-7] Cell Complete Medium is a ready-to-use medium that can be directly used for the culture of MCF7 [MCF-7] cells, which is carefully designed and optimized by Pricella's R&D team based on MEM, with NEAA basal medium. This high-quality complete medium has been strictly verified by MCF7 [MCF-7] cells which could promote cell proliferation and maintain cell properties.

Manufacturer: Procell System

MCDB is a low-protein and serum-free culture medium designed for specific cells. MCDB 131 is a kind of MCDB medium designed and developed by Kendler and Ham. It was originally used for culturing human microvascular endothelial cells (HMVEC). In addition to the use of MCDB 131 medium for endothelial cells, it can also be used for other cell types, such as liver cells, smooth muscle cells, and cardiomyocytes. MCDB 131 medium is not used in traditional medium because it contains trace elements, putrescine, adenine, thymine and higher concentrations of amino acids and vitamins. It is often used with EGF, hydrocortisone, glutamine and low-concentration serum together, and the added concentration of serum must be determined by cell type.

Manufacturer: Procell System

NCTC clone 929 Cell Complete Medium is a ready-to-use medium that can be directly used for the culture of NCTC clone 929 cells, which is carefully designed and optimized by Pricella's R&D team based on MEM, with NEAA basal medium. This high-quality complete medium has been strictly verified by NCTC clone 929 cells which could promote cell proliferation and maintain cell properties.

Manufacturer: Procell System

The DMEM/F12 medium is based on the DMEM medium, adding more nutritious elements from F12 medium, which contains a variety of trace elements, and is widely used in the culture of many mammalian cells. At the same time, DMEM/F12 medium is often used as the basis for the development of serum-free medium. It is also suitable for mammalian cell culture and clone density culture under low serum content. This product contains many kinds of amino acids, vitamins, inorganic salts and other ingredients for cell culture, but does not contain protein, lipids or any growth factors. Therefore, the product should be used with serum or serum-free additives.

Manufacturer: Procell System

L-alanyl-L-glutamine is an advanced cell culture additive and as a substitute for L-glutamine. L-glutamine is a necessary nutrient element for cells. But it is unstable in solution and can be degraded spontaneously to form ammonia and pyroglutamic acid and ammonia is harmful to cells. L-alanyl-L-glutamine is stable in aqueous solution and does not degrade spontaneously. The mechanism used by the cell is that the cell gradually releases a peptidase into the culture medium during cell culture. The peptidase can hydrolyze L-alanyl-L-glutamine into L-alanine and L-glutamine. L-alanine and L-glutamine can be absorbed and utilized by cells. This not only increases the utilization rate of L-glutamine, but also does not produce excess ammonia, which is more beneficial to cell growth.

Manufacturer: Procell System

MEMα was developed on the basis of MEM medium. Compared with MEM medium, NEAA, sodium pyruvate, zinc sulfate, VB12, biotin and ascorbic acid were added. It is widely used in suspension and adherent cell culture of various mammals. MEMα medium without nucleoside and deoxynucleoside is often used as medium for DG44 and other DHFR- cells. This product contains many kinds of amino acids, vitamins, inorganic salts and other ingredients for cell culture, but does not contain protein, lipids or any growth factors. Therefore, the product should be used with serum or serum-free additives.

Manufacturer: Procell System

NK-92MI Cell Complete Medium is a ready-to-use medium that can be directly used for the culture of NK-92MI cells, which is carefully designed and optimized by Pricella's R&D team based on MEMα basal medium. This high-quality complete medium has been strictly verified by NK-92MI cells which could promote cell proliferation and maintain cell properties.

Manufacturer: Procell System

RPMI-1640 is an improved McCoy's 5A medium, which uses bicarbonate buffer system. RPMI-1640 medium was originally designed for lymphocyte culture. Nowadays, it has been widely used in the culture of normal cells and cancer cells, especially suspension cells, which is one of the most widely used media. RPMI-1640 medium contains many kinds of amino acids, vitamins, inorganic salts and other ingredients for cell culture, but does not contain protein, lipids or any growth factors. Therefore, the product should be used serum or serum-free additives.

Manufacturer: Procell System

Normal saline refers to the sodium chloride solution commonly used in physiological experiments or clinical practice, whose osmotic pressure is equal to that of animals or human body. It is commonly used as a fluid supplement and is also often used in vitro. Different types of animals require different concentrations of normal saline. Mammals use 0.9% normal saline, birds use 0.75% normal saline, and amphibians use 0.65% normal saline.

Manufacturer: Procell System

RPMI-1640 is an improved McCoy's 5A medium, which uses bicarbonate buffer system. RPMI-1640 medium was originally designed for lymphocyte culture. Nowadays, it has been widely used in the culture of normal cells and cancer cells, especially suspension cells, which is one of the most widely used media. RPMI-1640 medium contains many kinds of amino acids, vitamins, inorganic salts and other ingredients for cell culture, but does not contain protein, lipids or any growth factors. Therefore, the product should be used serum or serum-free additives.

Manufacturer: Procell System

HMC3 Cell Complete Medium is a ready-to-use medium that can be directly used for the culture of HMC3 cells, which is carefully designed and optimized by Pricella's R&D team based on MEM, with NEAA basal medium. This high-quality complete medium has been strictly verified by HMC3 cells which could promote cell proliferation and maintain cell properties.

Manufacturer: Procell System

AGS Cell Complete Medium is a ready-to-use medium that can be directly used for the culture of AGS cells, which is carefully designed and optimized by Pricella's R&D team based on Ham's F-12 basal medium. This high-quality complete medium has been strictly verified by AGS cells which could promote cell proliferation and maintain cell properties.

Manufacturer: Procell System

HCT 116 Cell Complete Medium is a ready-to-use medium that can be directly used for the culture of HCT 116 cells, which is carefully designed and optimized by Pricella's R&D team based on McCoy's 5A basal medium. This high-quality complete medium has been strictly verified by HCT 116 cells which could promote cell proliferation and maintain cell properties.

Manufacturer: Procell System

Hela Cell Complete Medium is a ready-to-use medium that can be directly used for the culture of Hela cells, which is carefully designed and optimized by Pricella's R&D team based on MEM, with NEAA basal medium. This high-quality complete medium has been strictly verified by Hela cells which could promote cell proliferation and maintain cell properties.

Manufacturer: Procell System

Balanced Salt Solution (Physiological Solution) have the properties of buffer solution (regulate pH), normal saline (maintain osmotic pressure) and culture medium (provide nutrition). It can meet the basic needs of survival and metabolism of tissues, organs or cells in vitro. A small amount of phenolic red was added to some equilibrium salt solutions to indicate the pH change of the solution. Phosphate Buffered Saline (PBS) is one of the most widely used equilibrium salt solutions in biochemistry. The main components are Na2HPO4、KH2PO4、NaCl and KCl. PBS buffers are often used to rinse tissue or cells, to transport cells or tissues, to prepare other reagents, to dilute the cells when counting the cells.

Manufacturer: Procell System

DMEM (Dulbecco's Modified Eagle Medium) was developed on the basis of MEM medium. Compared with MEM medium, the content of amino acid increased by 2 times, the content of vitamin increased by 4 times, and the content of non-essential amino acid, trace iron ion and sodium pyruvate were increased by 4 times. The glucose content of DMEM medium was originally designed as 1000 mg/L (low Glucose type), and then developed into 4500 mg/L (high Glucoser type), which has been widely used in cell culture. DMEM (Low glucose) contains many kinds of amino acids, vitamins, inorganic salts and other ingredients for cell culture, but does not contain protein, lipids or any growth factors, so the product should be used with serum or serum-free additives.

Manufacturer: Procell System

MRC-5 Cell Complete Medium is a ready-to-use medium that can be directly used for the culture of MRC-5 cells, which is carefully designed and optimized by Pricella's R&D team based on MEM, with NEAA basal medium. This high-quality complete medium has been strictly verified by MRC-5 cells which could promote cell proliferation and maintain cell properties.

Manufacturer: Procell System

The Mouse Bone Marrow Mesenchymal Stem Cells Osteogenic Differentiation Medium is specifically designed to facilitate osteogenic differentiation. The differentiation reagent's formula has been tailored to the unique characteristics of mouse bone marrow mesenchymal stem cells, thereby enhancing their osteogenic differentiation potential. This product is exclusively intended for scientific research. It must not be used for diagnostic, therapeutic, clinical, or any other purposes.

Manufacturer: Procell System

Vitamins is a kind of organic compound necessary to maintain normal life activities. Vitamin has little content in body, but plays an important role in human growth, metabolism and development.Most of the vitamins that animal cells cannot produce on their own or in insufficient quantities to meet the needs of the cells themselves must be supplied from outside.MEM Vitamin Solution, which contains 50 times the Vitamin content of MEM medium, contains choline chloride, calcium d-pantothenate, folate, niacinamide, pyridoxal hydrochloride, riboflavin, thiamine hydrochloride and inositol. It is one of the commonly used additives in cell culture.

Manufacturer: Procell System

NIH/3T3 Cell Complete Medium is a ready-to-use medium that can be directly used for the culture of NIH/3T3 cells, which is carefully designed and optimized by Pricella's R&D team based on DMEM basal medium. This high-quality complete medium has been strictly verified by NIH/3T3 cells which could promote cell proliferation and maintain cell properties.

Manufacturer: Procell System

LX-2 Cell Complete Medium is a ready-to-use medium that can be directly used for the culture of LX-2 cells, which is carefully designed and optimized by Pricella's R&D team based on DMEM basal medium. This high-quality complete medium has been strictly verified by LX-2 cells which could promote cell proliferation and maintain cell properties.

Manufacturer: Procell System

Trypsin is a serine hydrolase that can cut the base-side segment of the lysine and arginine residue in the polypeptide chain, hydrolyze the protein between the cells, and destroy the connection between the cells, so that the tissue or the adherent cells are dispersed into a single cell. The activity of the trypsin is related to the characteristics of the tissue or the cells, the concentration of the trypsin, the temperature and the time of action. At pH 8.0 and 37℃, the effect of the trypsin is the best. Therefore, the concentration of trypsin, the temperature and the time of the pancreatic enzyme should be grasped to avoid the excessive damage of the cells. In general, the frequently-used working concentration of the trypsin is 0.25%, while the concentration of trypsin for the semi-adherent cells or the cells is 0.05%. Since EDTA is able to bind Ca2+ and Mg2+ to destroy the cell connection to promote the dissociation of the cells, a certain amount of EDTA is often added to the trypsin solution to enhance the dissociation effect.

Manufacturer: Procell System

The Rat Bone Marrow Mesenchymal Stem Cells Osteogenic Differentiation Medium is specifically designed to facilitate osteogenic differentiation. The differentiation reagent's formula has been tailored to the unique characteristics of rat bone marrow mesenchymal stem cells, thereby enhancing their osteogenic differentiation potential. This product is exclusively intended for scientific research. It must not be used for diagnostic, therapeutic, clinical, or any other purposes.

Manufacturer: Procell System

AC16 Cell Complete Medium is a ready-to-use medium that can be directly used for the culture of MCF-12A cells, which is carefully designed and optimized by Pricella's R&D team based on DMEM/F12, with HEPES basal medium. This high-quality complete medium has been strictly verified by AC16 cells which could promote cell proliferation and maintain cell properties.

Manufacturer: Procell System

Hepa 1-6 Cell Complete Medium is a ready-to-use medium that can be directly used for the culture of Hepa 1-6 cells, which is carefully designed and optimized by Pricella's R&D team based on DMEM（with Sodium Pyruvate） basal medium. This high-quality complete medium has been strictly verified by Hepa 1-6 cells which could promote cell proliferation and maintain cell properties.

Manufacturer: Procell System

The DMEM/F12 medium is based on the DMEM medium, adding more nutritious elements from F12 medium, which contains a variety of trace elements, and is widely used in the culture of many mammalian cells. At the same time, DMEM/F12 medium is often used as the basis for the development of serum-free medium. It is also suitable for mammalian cell culture and clone density culture under low serum content. This product contains many kinds of amino acids, vitamins, inorganic salts and other ingredients for cell culture, but does not contain protein, lipids or any growth factors. Therefore, the product should be used with serum or serum-free additives.

Manufacturer: Procell System

HuH-7 Cell Complete Medium is a ready-to-use medium that can be directly used for the culture of HuH-7 cells, which is carefully designed and optimized by Pricella's R&D team based on DMEM basal medium. This high-quality complete medium has been strictly verified by HuH-7 cells which could promote cell proliferation and maintain cell properties.

Manufacturer: Procell System

PANC-1 Cell Complete Medium is a ready-to-use medium that can be directly used for the culture of PANC-1 cells, which is carefully designed and optimized by Pricella's R&D team based on DMEM basal medium. This high-quality complete medium has been strictly verified by PANC-1 cells which could promote cell proliferation and maintain cell properties.

Manufacturer: Procell System

RPMI-1640 is an improved McCoy's 5A medium, which uses bicarbonate buffer system. RPMI-1640 medium was originally designed for lymphocyte culture. Nowadays, it has been widely used in the culture of normal cells and cancer cells, especially suspension cells, which is one of the most widely used media. The ATCC-modified RPMI-1640 adds sodium pyruvate and HEPES to increase the sugar content and decrease the Sodium bicarbonate content. RPMI-1640 medium contains many kinds of amino acids, vitamins, inorganic salts and other ingredients for cell culture, but does not contain protein, lipids or any growth factors. Therefore, the product should be used serum or serum-free additives.

Manufacturer: Procell System

DMEM (Dulbecco's Modified Eagle Medium) was developed on the basis of MEM medium. Compared with MEM medium, the content of amino acid increased by 2 times, the content of vitamin increased by 4 times, and the content of non-essential amino acid, trace iron ion and sodium pyruvate were increased by 4 times. The glucose content of DMEM medium was originally designed as 1000 mg/L (low Glucose type), and then developed into 4500 mg/L (high Glucoser type), which has been widely used in cell culture. DMEM medium without glucose can be added to the concentration of glucose at will according to the research needs, which is convenient and quick. DMEM (glucose free) contains many kinds of amino acids, vitamins, inorganic salts and other ingredients for cell culture, but does not contain protein, lipids or any growth factors, so the product should be used with serum or serum-free additives.

Manufacturer: Procell System

Neuro-2a [N2a] Cell Complete Medium is a ready-to-use medium that can be directly used for the culture of Neuro-2a [N2a] cells, which is carefully designed and optimized by Pricella's R&D team based on MEM, with NEAA basal medium. This high-quality complete medium has been strictly verified by Neuro-2a [N2a] cells which could promote cell proliferation and maintain cell properties.

Manufacturer: Procell System

TNM-FH medium is an improved Grace's insect medium. After proper supplementation, it can be used for the culture of various Lepidoptera insect cells. TNM-FH medium contains amino acids, vitamins, inorganic salts and other ingredients required for various types of cell culture, but does not contain protein, lipids or any growth factors, so this product needs to be used with serum or serum-free additives.

Manufacturer: Procell System

Interleukin-2 (IL-2) is an α-helix cytokine with a molecular weight of 15 kDa. It is a lymphatic factor with strong immune regulation and is produced by T cells in response to antigenicity or mitogenic stimulation. This lymph factor promotes the long-term growth of activated T cells and related cell types. IL-2 plays a role in the activation and proliferation of NK cells, induces the secretion of γ-interferon and B cell growth factor, and regulates the expression of IL-2 receptor.

Manufacturer: Procell System

The MC3T3-E1 Subclone 14 Cells Osteogenic Differentiation Medium is specifically designed to facilitate osteogenic differentiation. The differentiation reagent's formula has been tailored to the unique characteristics of MC3T3-E1 Subclone 14 cells, thereby enhancing their osteogenic differentiation potential. This product is exclusively intended for scientific research. It must not be used for diagnostic, therapeutic, clinical, or any other purposes.

Manufacturer: Procell System

Neurobasal medium is a basic neuron culture medium that does not contain L-glutamine and requires the addition of B-27 neuron Supplement with vitamin A , 50 × (product number: PB180637). It is suitable for short-term or long-term maintenance and cultivation of neuronal cells in newborn and mature mice.

Manufacturer: Procell System

MEMα was developed on the basis of MEM medium. Compared with MEM medium, NEAA, sodium pyruvate, zinc sulfate, VB12, biotin and ascorbic acid were added. It is widely used in suspension and adherent cell culture of various mammals. MEMα medium without nucleoside and deoxynucleoside is often used as medium for DG44 and other DHFR- cells. This product contains many kinds of amino acids, vitamins, inorganic salts and other ingredients for cell culture, but does not contain protein, lipids or any growth factors. Therefore, the product should be used with serum or serum-free additives.

Manufacturer: Procell System

Nanobacteria and their decomposition complexes are the common contaminant in cell cultures that co-exists with cells. Antibiotics are usually ineffective. Nanobacteria grows competitively with cells, which is unfavorable to cell growth, and in severe cases causes cell death. At present, many cells are contaminated with nanobacteria, which has a great impact on cell culture and subsequent experiments. The common characteristics of cells contaminated by nanobacteria are as follows: (1) The medium is not turbid, but when the cells are observed under a microscope, there are many "small black spots" around the cells or in the culture medium. With the extension of culture time, the "small black spots" gradually increase, and they cannot be removed by changing the culture medium or washing the cells. (2) The cells contaminated by the "small black spots" consume fast nutrients and require frequent replacement of the culture medium. (3) Nanobacteria-contaminated cells grow slowly, have poor cell states, and are severely vacuolated. They may even cause changes in cell morphology. Therefore, it is of great significance to clean up nanobacteria contamination in cell culture. Anti-Nanobacteria media is a new generation product developed by our team on the basis of Biomocin products. It contains special ingredients to remove nanobacteria. This product has been tested on hundreds of cells and verified by long-term experiments. It is harmless to cells and has a significant effect on removing and inhibiting nanobacteria.