Nimble Juice (Speedy Protein Gel Stain)
Description
Nimble Juice is a fast and sensitive fluorescent
dye for visualization and quantitation of proteins separated by 1-D
or 2-D SDS-PAGE. It comes as a 100x stock solution that is simply
diluted with water by the user to its working concentration. Nimble
Juice is normally low fluorescent but emits strong fluorescence (bright
golden color) as bound to proteins. The staining procedure is a simple
two-step protocol (fix and stain) that can be completed in as little as
30 minutes. Gels to be stained are fixed with ethanol/acetic acid
solution prior to staining with Nimble Juice solution. A destain step is
not normally recommended, but may be employed to reduce background,
simply by agitating the gel in water for 1-5 minutes. Gels stained with
Nimble Juice fluorescent gel stain may be directly visualized with a
variety of different UV-based fluorescence imaging systems. The maximum
emission wavelength of protein-bound Nimble Juice is near 570 nm. Nimble
Juice gives exceptional sensitivity and wide dynamic range for protein
detection. The bound Nimble Juice dye is easily removed from the protein
by immersing the gel in sufficient water, thus it is well compatible
with subsequent enzymatic digestion and mass spectrometry for
proteomics applications. Stained gels may be stored in stain solution in
the dark at 2-8°C; imaging sensitivity might be moderately enhanced
after 4°C storage of the stained gel.
Size: 10 ml (100x)
Storage
The product is stable for at least 6 months when stored at 2-8°C.
Avoid exposure to temperatures greater than 37°C and protect from light.
Ordering Information
| Cat.No. | Pack Size |
| SJ001-0010 | 10 ml (100x) |
