Elabscience Assay Kits

Manufacturer: Elabscience

Lipoxygenase (LOX) is an iron-containing oxidoreductase found in plants and animals. The detection principle of this kit is as follows: LOX catalyzes the oxidation of arachidonic acid to produce fluorescence products, and the enzyme activity of LOX can be calculated according to the change of fluorescence value of fluorescence products at the excitation wavelength of 507 nm and the emission wavelength of 547 nm within a unit time.

Manufacturer: Elabscience

Mitochondrial oxidative phosphorylation consumes oxygen to produce ATP, which provides energy for cell growth. Therefore, detection of cellular oxygen consumption is a key indicator of mitochondrial function. The kit provides a fluorescent probe, which is sensitive to oxygen. The fluorescence of the probe increases with the decrease of oxygen in a closed environment, and the oxygen consumption rate of cells is judged by detecting the change of the fluorescence value.

Manufacturer: Elabscience

Lipid peroxides (LPO) are substances produced by the action of reactive oxygen species (ROS) on unsaturated fatty acids. The LPO produced by the reaction will continue to oxidize the liposomes, thereby accumulating LPO in a chain reaction. Excess LPO decomposes into highly active aldehydes such as Acrolein, Malondialdehyde (MDA), and 4-hydroxy-2-nonenal (4-NHE), causing cell toxicity and cell death. This kit provides a fluorescent probe that can detect LPO production. This fluorescent probe can react with the lipid free radicals in the lipid peroxidation pathway, so as to detect the level of lipid peroxidation in cells. The probe fluoresces red under normal conditions through photoexcitation, and the fluorescence changes from red to green with the process of lipid peroxidation. Through the enhancement of green fluorescence intensity, the production of LPO can be detected with high sensitivity.

Manufacturer: Elabscience

Mitochondrial complex V is also known as F0F1-ATP synthase. ATP is hydrolyzed by F0F1-ATP synthase to produce ADP, and ADP converts NADH into oxidized coenzyme I (NAD) after enzyme conversion reaction. The activity of mitochondrial complex V can be quantified by measure the change OD value at 340 nm.

Manufacturer: Elabscience

Under the activation of the activator, IDH converts isocitrate into α-ketoglutaric acid. Meanwhile, NAD+ is reduced to NADH, which under the action of hydrogen transmitter, transfer electrons to WST-8 to produce the yellow product. The activity of NAD-IDH can be calculated by measuring the change of absorbance value at 450 nm.

Manufacturer: Elabscience

SDH catalyzes the dehydrogenation of succinate to fumarate, with electron transport materials transferring electrons to 2, 6-dichlorophenol indiophenol (DCPIP). Then, the reduced DCPIP is reduced to the oxidized DCPIP, which has a characteristic absorption peak at 600 nm. Therefore, the activity of SDH can be quantified by measure the change OD value at 600 nm.

Manufacturer: Elabscience

Malate dehydrogenase widely exists in animals, plants, bacteria and other organisms, is one of the key enzymes in tricarboxylic acid cycle, catalyze between malic acid and oxaloacetic acid reversible conversion. MDH can be divided into NAD-dependent MDH and NADP-dependent MDH according to different coenzyme specificity. NAD-MDH usually exists in the cytoplasm and mitochondria of bacteria and eukaryotic cells. NAD-MDH catalyzes the conversion of substrate malic acid and NAD to oxaloacetic acid and NADH. NADH makes WST-8 orange under the action of electron coupling agent. The activity of NAD-MDH can be calculated by measuring the change of absorbance value at 450 nm.

Manufacturer: Elabscience

The Citric Acid (CA) Colorimetric Assay Kit (Catalog No. E-BC-K351-M) from Elabscience is designed to measure citric acid content in various samples, including tissues and mitochondria. This assay is essential for studying the citric acid cycle, a fundamental component of cellular energy metabolism.

Manufacturer: Elabscience

The Mitochondrial Complex III (Coenzyme Q-Cytochrome c Reductase) Activity Assay Kit (Catalog No. E-BC-K151-M) from Elabscience is designed to measure the activity of mitochondrial Complex III, also known as cytochrome c reductase. This enzyme plays a crucial role in the mitochondrial respiratory chain by facilitating the transfer of electrons from coenzyme Q to cytochrome c, contributing to cellular energy production.