Malic Acid Colorimetric Assay Kit
Cat No.: E-BC-K905-M | Size: 96T, 48T
Detection Principle | L-malic acid, also known as 2-hydroxysuccinic acid, is widely present in living organisms. It has two stereoisomers and is one of the members of the tricarboxylic acid cycle, an important metabolic cycle in living organisms. The detection principle of this kit is that the enzyme catalyzes malic acid to produce oxaloacetic acid. At the same time, under the action of electronic coupler, the chromogenic agent is reduced to produce orange yellow product, which has the maximum absorption peak at about 450 nm. |
Sample Type | Serum,Plasma,Tissue,Cell |
Detection Method | Colorimetric method |
Detection Instrument | Microplate reader (445-465 nm, optimum wavelength: 450 nm) |
Research Area | Tricarboxylic Acid(TCA)Cycle, Energy Metabolism |
Other Reagents Required | Normal saline (0.9% NaCl) |
Storage | This product can be stored at -20°C for 12 months with shading light. |
Valid Period | 12 months |
Sensitivity | 0.003 mmol/L |
Detection Range | 0.003-0.5 mmol/L |
Precision | inter-assay CV: 4.6-5.9% | intra-assay CV: 2.6-3.6% |