8-OHdG(8-Hydroxydeoxyguanosine) ELISA Kit
Cat No.: EU2548 | Size: 48T & 96T
| Synonyms: | 8-OHdG ELISA Kit, 8-Hydroxydeoxyguanosine ELISA Kit | 
| Detection Method: | Competitive ELISA, Coated with Antigen | 
| Application: | This kit is based on Competitive-ELISA detection method and takes 2h assay time. The microplate provided in this kit has been precoated with 8-OHdG. 8-OHdG in the sample or standard competes with a fixed amount of biotin-labeled 8-OHdG for sites on a pre-coated antibody specific to 8-OHdG. Free components are washed off. After adding HRP-Streptavidin Conjugate (SABC), biotin specifically binds with streptavidin to form immune complexes. Wash off unbound conjugate. Add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Compare OD450 value of sample with the standard curve through curve fitting software to determine the concentration of 8-OHdG in the sample. The concentration of the target substance is inversely proportional to the OD450 value. | 
| Range: | 1.563-100ng/ml | 
| Sensitivity: | 0.938ng/ml | 
| Species: | Universal | 
| Storage: | 2-8°C(Sealed), Don't cryopreserve. | 
| Note: | For Research use only | 
Please Note: Manuals on the web are for your reference only, specifications are subject to the delivery manuals.

 
               
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