Rat IL-1β(Interleukin 1 Beta) ELISA Kit
Cat No.: ER1094 | Size: 48T & 96T
| Synonyms: | Interleukin-1 beta ELISA Kit, IL-1 beta ELISA Kit, Catabolin ELISA Kit, IL1B ELISA Kit, IL1F2 ELISA Kit | 
| Detection Method: | Sandwich ELISA, Double Antibody | 
| Application: | This kit is based on Double antibody-Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti IL-1β antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody. Then, it binds with IL-1β bound to precoated antibody. Wash unbound components and add HRP-Streptavidin Conjugate (SABC). Wash unbound components again and add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Calculate the concentration of IL-1β in the sample by plotting standard curve. The concentration of the target substance is proportional to the OD450 value. | 
| Range: | 31.25-2000pg/ml | 
| Sensitivity: | 18.75pg/ml | 
| Species: | Rat | 
| Storage: | 2-8°C(Sealed), Don't cryopreserve. | 
| Note: | For Research use only | 
Please Note: Manuals on the web are for your reference only, specifications are subject to the delivery manuals.

 
               
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