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Manufacturer: Elabscience
Balanced Salt Solution (Physiological Solution) have the properties of buffer solution (regulate pH), normal saline (maintain osmotic pressure) and culture medium (provide nutrition). It can meet the basic needs of survival and metabolism of tissues, organs or cells in vitro. A small amount of phenolic red was added to some equilibrium salt solutions to indicate the pH change of the solution. Dulbecco's Phosphate Buffered Saline (DPBS) is one of the most widely used equilibrium salt solutions in biochemistry. The main components are NaCl, KCl, KH2PO4 and Na2HPO4. DPBS can be divided into two types according to whether contain calcium and magnesium. Different from conventional PBS, DPBS phosphate content was slightly lower. DPBS was mainly used for embryological research.
Manufacturer: Elabscience
McCoy's 5A medium was initially used specifically for the culture of Novikoff Hepatoma cells. Unlike other medium, McCoy's 5A medium contains reduced glutathione, bacterial peptone and high concentrations of glucose. McCoy's 5A is widely used in the culture of primary cells, such as bone marrow, skin, gingiva, kidney, spleen, lung, rat embryo, omentum and so on. In addition, McCoy's 5A medium is also used for tissue biopsy culture, the establishment of cell line, and the culture of some lymphocytes and more difficult cells. This product contains many kinds of amino acids, vitamins, inorganic salts and other ingredients for cell culture, but does not contain protein, lipids or any growth factors. Therefore, the product should be used with serum or serum-free additives.
Manufacturer: Elabscience
Trypsin is a serine hydrolase that can cut the base-side segment of the lysine and arginine residue in the polypeptide chain, hydrolyze the protein between the cells, and destroy the connection between the cells, so that the tissue or the adherent cells are dispersed into a single cell. The activity of the trypsin is related to the characteristics of the tissue or the cells, the concentration of the trypsin, the temperature and the time of action. At pH 8.0 and 37℃, the effect of the trypsin is the best. Therefore, the concentration of trypsin, the temperature and the time of the pancreatic enzyme should be grasped to avoid the excessive damage of the cells. In general, the frequently-used working concentration of the trypsin is 0.25%, while the concentration of trypsin for the semi-adherent cells or the cells is 0.05%. Since EDTA is able to bind Ca2+ and Mg2+ to destroy the cell connection to promote the dissociation of the cells, a certain amount of EDTA is often added to the trypsin solution to enhance the dissociation effect.
Manufacturer: Elabscience
Penicillin-streptomycin solution mixture is the most commonly used antibiotic to prevent microbial contamination in vitro. Penicillin can interfere with the synthesis of bacterial cell wall, especially for Gram-positive bacteria. Streptomycin could bind to ribosomal ribosome for 30S and inhibit the synthesis of bacterial protein. It was effective for Gram-negative bacteria and Gram-positive bacteria, but especially for Gram-negative bacteria. The combined use of penicillin and streptomycin can prevent most bacterial contamination, but penicillin solution is sensitive to temperature and pH, easy to degrade at room temperature, and needs cryopreservation, the stability of penicillin solution is the most stable when pH is 6.0-6.5. Streptomycin was relatively stable, and pH 5.0-7.5 was the most stable.
Manufacturer: Elabscience
RAW 264.7 Cell Complete Medium is a ready-to-use medium that can be directly used for the culture of RAW 264.7 cells, which is carefully designed and optimized by Pricella's R&D team based on DMEM basal medium. This high-quality complete medium has been strictly verified by RAW 264.7 cells which could promote cell proliferation and maintain cell properties.
Manufacturer: Elabscience
The Ham's F-12K medium increases the concentration of amino acids and pyruvic acid on the basis of Ham's F-12, reduces the concentration of glucose, and modifies the composition and content of the salt.The Ham's F-12K medium was initially designed to culture primary human hepatocytes and differentiated cells of rats and chickens. The Ham's F-12K medium contains many kinds of amino acids, vitamins, inorganic salts and other ingredients for cell culture, but does not contain protein, lipids or any growth factors. Therefore, the product should be used with serum or serum-free additives.
Manufacturer: Elabscience
In vitro cell culture, in order to preserve the biological activity of cells for a long time, the cells must be cryopreserved, and resuscitated when necessary. At present, the most commonly used method for cell cryopreservation is liquid nitrogen method. In the process, add an appropriate amount of protective agent to slowly cool the cells to the specified temperature range, so as to achieve the purpose of protecting the cells. The Serum-free Cell Freezing Medium is a special cryopreservation product developed by Pricella for hundreds of types of cells by continuously optimizing experimental conditions for cell cryopreservation and resuscitation in the long-term cell research. The product adds cell sedimentation stabilizer, which can delay the sedimentation rate of cells in the process of cryopreservation, prevent cells from squeezing each other and affect the effect of cell cryopreservation. In addition, a variety of cryoprotectants, such as cell membrane protectors, permeable intracellular membrane protectors, and non-permeable cell protectors, are added. These components combine with water molecules in the solution to hydrate and weaken the crystallization process of water, increasing the viscosity of the solution and reducing the formation of ice crystals, which can greatly reduce the damage of ice crystals to cells during cryopreservation, and effectively improve the survival rate of cell after resuscitation. The product formula is clear, does not contain serum or animal derived protein, which can reduce comtamination risk from bacteria, viruses and mycoplasma, and ensure the safety of frozen cells. It is not only suitable for conventional cell lines, primary cells, but also for serum-free culture cells and protein-expressing cells. Compared with traditional cryopreservation media, there is no need for tedious and time-consuming gradient freezing steps, and no need for expensive program cooling equipment. Cells can be directly resuspended and placed at -80℃, and transferred to liquid nitrogen the next day to complete the entire process, saving a lot of time and energy.
Manufacturer: Elabscience
MEMα was developed on the basis of MEM medium. Compared with MEM medium, NEAA, sodium pyruvate, zinc sulfate, VB12, biotin and ascorbic acid were added. It is widely used in suspension and adherent cell culture of various mammals. MEMα medium without nucleoside and deoxynucleoside is often used as medium for DG44 and other DHFR- cells. This product contains many kinds of amino acids, vitamins, inorganic salts and other ingredients for cell culture, but does not contain protein, lipids or any growth factors. Therefore, the product should be used with serum or serum-free additives.
Manufacturer: Elabscience
Trypsin is a serine hydrolase that can cut the base-side segment of the lysine and arginine residue in the polypeptide chain, hydrolyze the protein between the cells, and destroy the connection between the cells, so that the tissue or the adherent cells are dispersed into a single cell. The activity of the trypsin is related to the characteristics of the tissue or the cells, the concentration of the trypsin, the temperature and the time of action. At pH 8.0 and 37℃, the effect of the trypsin is the best. Therefore, the concentration of trypsin, the temperature and the time of the pancreatic enzyme should be grasped to avoid the excessive damage of the cells. In general, the frequently-used working concentration of the trypsin is 0.25%, while the concentration of trypsin for the semi-adherent cells or the cells is 0.05%. Since EDTA is able to bind Ca2+ and Mg2+ to destroy the cell connection to promote the dissociation of the cells, a certain amount of EDTA is often added to the trypsin solution to enhance the dissociation effect.