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Manufacturer: Elabscience

Balanced Salt Solution (Physiological Solution) have the properties of buffer solution (regulate pH), normal saline (maintain osmotic pressure) and culture medium (provide nutrition). It can meet the basic needs of survival and metabolism of tissues, organs or cells in vitro. A small amount of phenolic red was added to some equilibrium salt solutions to indicate the pH change of the solution. Hank's Balanced Salt Solution (HBSS) is one of the commonly used phosphate buffers in cell separation or culture. The main components are NaCl、KCl、KH2PO4、Na2HPO4、NaHCO3 and glucose. HBSS is used for the washing of cells before passage and tissue dissociation, preparation of cell dissociation reagent, transport of cell or tissue, dilution of cells, etc.

Manufacturer: Elabscience

Trypsin is a serine hydrolase that can cut the base-side segment of the lysine and arginine residue in the polypeptide chain, hydrolyze the protein between the cells, and destroy the connection between the cells, so that the tissue or the adherent cells are dispersed into a single cell. The activity of the trypsin is related to the characteristics of the tissue or the cells, the concentration of the trypsin, the temperature and the time of action. At pH 8.0 and 37℃, the effect of the trypsin is the best. Therefore, the concentration of trypsin, the temperature and the time of the pancreatic enzyme should be grasped to avoid the excessive damage of the cells. In general, the frequently-used working concentration of the trypsin is 0.25%, while the concentration of trypsin for the semi-adherent cells or the cells is 0.05%. Since EDTA is able to bind Ca2+ and Mg2+ to destroy the cell connection to promote the dissociation of the cells, a certain amount of EDTA is often added to the trypsin solution to enhance the dissociation effect.

Manufacturer: Elabscience

Ham's F-12 Nutrient Mixture was designed by Ham in 1969 based on Ham's F-10 nutrient mixture, which was originally used for serum-free culture of CHO cells. Ham's F-12 is often used as the basic culture medium for serum free culture. At low serum levels, it is especially suitable for single cell culture and clonal culture. After adding serum, it is also widely used in the culture of cancer cells and primary cells, such as rat hepatocytes, rat prostate epithelial cells, chondrocytes, rat myoblasts, and chicken embryonic cells. In addition, the DMEM/F12 medium obtained by mixing Ham's F-12 in equal volume with DMEM is more nutritious and widely used.

Manufacturer: Elabscience

The buffer system of Leibovitz's L-15 medium is composed of phosphate and free amino acids, instead of the traditional sodium bicarbonate buffer system. Meanwhile, the substitution of galactose and sodium pyruvate for glucose prevents prevents the formation of acidic metabolic by-products, helps to maintain the stable pH of the medium. It is suitable for cell culture in a non-CO2 balanced environment. Leibovitz's L-15 medium is suitable for the culture of monkey kidney cells and HEP-2, the isolation of primary cells (such as embryonic and adult tissues), the culture of various viruses and the culture of neurons. Leibovitz's L-15 medium contains many kinds of amino acids, vitamins, inorganic salts and other ingredients for cell culture, but does not contain protein, lipids or any growth factors. Therefore, the product should be used with serum or serum-free additives.

Manufacturer: Elabscience

AML12 Cell Complete Medium is a ready-to-use medium that can be directly used for the culture of AML12 cells, which is carefully designed and optimized by Pricella's R&D team based on DMEM/F12 basal medium. This high-quality complete medium has been strictly verified by AML12 cells which could promote cell proliferation and maintain cell properties.

Manufacturer: Elabscience

BV2 Cell Complete Medium is a ready-to-use medium that can be directly used for the culture of BV2 cells, which is carefully designed and optimized by Pricella's R&D team based on DMEM basal medium. This high-quality complete medium has been strictly verified by BV2 cells which could promote cell proliferation and maintain cell properties.

Manufacturer: Elabscience

SH-SY5Y Cell Complete Medium is a ready-to-use medium that can be directly used for the culture of SH-SY5Y cells, which is carefully designed and optimized by Pricella's R&D team based on MEM/F12 basal medium. This high-quality complete medium has been strictly verified by SH-SY5Y cells which could promote cell proliferation and maintain cell properties.

Manufacturer: Elabscience

Insulin is a double-stranded (α, β) peptide hormone secreted by islet β cells. Insulin functions of different species (human, cattle, sheep, pig, etc.) are generally the same, and the composition is slightly different. Insulin is currently known the only hormone in the body that lowers blood sugar, and the only hormone that simultaneously promotes glycogen, fat, and protein synthesis. Insulin plays an important role in many cellular activities. It is a commonly used substance in mammalian cell culture. Insulin can accelerate the use of glucose and inhibit the production of glucose, inhibit the decomposition of fat, promote the synthesis and storage of fat, promote the synthesis of protein, prevents the breakdown of proteins, promotes potassium and magnesium ions to pass through the cell membrane into the cell, promotes the synthesis of deoxyribonucleic acid (DNA), ribonucleic acid (RNA) and adenosine triphosphate (ATP). Recombinant human insulin, produced by E.coli expression system by Elabscience, has a molecular weight of about 5800 Da and has the same structure as human insulin of natural origin. It is often used as an auxiliary component in cell culture. The recommended concentration is 1-10 μg/mL.

Manufacturer: Elabscience

MCF 10A Cell Complete Medium is a ready-to-use medium that can be directly used for the culture of MCF 10A cells, which is carefully designed and optimized by Pricella's R&D team based on DMEM/F12 basal medium. This high-quality complete medium has been strictly verified by MCF 10A cells which could promote cell proliferation and maintain cell properties.