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Manufacturer: Elabscience

The DMEM/F12 medium is based on the DMEM medium, adding more nutritious elements from F12 medium, which contains a variety of trace elements, and is widely used in the culture of many mammalian cells. At the same time, DMEM/F12 medium is often used as the basis for the development of serum-free medium. It is also suitable for mammalian cell culture and clone density culture under low serum content. This product contains many kinds of amino acids, vitamins, inorganic salts and other ingredients for cell culture, but does not contain protein, lipids or any growth factors. Therefore, the product should be used with serum or serum-free additives.

Manufacturer: Elabscience

Nanobacteria and their decomposition complexes are the common contaminant in cell cultures that co-exists with cells. Antibiotics are usually ineffective. Nanobacteria grows competitively with cells, which is unfavorable to cell growth, and in severe cases causes cell death. At present, many cells are contaminated with nanobacteria, which has a great impact on cell culture and subsequent experiments. The common characteristics of cells contaminated by nanobacteria are as follows: (1) The medium is not turbid, but when the cells are observed under a microscope, there are many "small black spots" around the cells or in the culture medium. With the extension of culture time, the "small black spots" gradually increase, and they cannot be removed by changing the culture medium or washing the cells (2) The cells contaminated by the "small black spots" consume fast nutrients and require frequent replacement of the culture medium (3) Nanobacteria-contaminated cells grow slowly, have poor cell states, and are severely vacuolated. They may even cause changes in cell morphology. Therefore, it is of great significance to clean up nanobacteria contamination in cell culture.

Manufacturer: Elabscience

DMEM (Dulbecco's Modified Eagle Medium) was developed on the basis of MEM medium. Compared with MEM medium, the content of amino acid increased by 2 times, the content of vitamin increased by 4 times, and the content of non-essential amino acid, trace iron ion and sodium pyruvate were increased by 4 times. The glucose content of DMEM medium was originally designed as 1000 mg/L (low Glucose type), and then developed into 4500 mg/L (high Glucoser type), which has been widely used in cell culture. DMEM (Low glucose) contains many kinds of amino acids, vitamins, inorganic salts and other ingredients for cell culture, but does not contain protein, lipids or any growth factors, so the product should be used with serum or serum-free additives.

Manufacturer: Elabscience

RPMI-1640 is an improved Mccoy's 5A medium, which uses bicarbonate buffer system. RPMI-1640 medium was originally designed for lymphocyte culture. Nowadays, it has been widely used in the culture of normal cells and cancer cells, especially suspension cells, which is one of the most widely used media. RPMI-1640 medium contains many kinds of amino acids, vitamins, inorganic salts and other ingredients for cell culture, but does not contain protein, lipids or any growth factors. Therefore, the product should be used serum or serum-free additives.

Manufacturer: Elabscience

Mycoplasma is the smallest and simplest prokaryote found so far, with a diameter of 0.1-0.3 μm. It can easily pass through the filter membrane and enter the cell culture system. It has various forms such as sphere, rod, filamentous and branching. Usually attached to the surface of the cell membrane, there is no cell wall, so ordinary antibiotics have no effect on it. Mycoplasma contamination is widespread and often underestimated in cell cultures. Mycoplasma contamination can cause many problems, such as changes in cell growth rate, cell morphology, chromosome aberrations, cell membrane antigenicity changes, cell metabolism changes, reduced survival rate after cell resuscitation, and so on, which ultimately leads to inaccurate or incorrect experimental results. Therefore, it is of great significance to clean up mycoplasma contamination in cell culture.

Manufacturer: Elabscience

THP-1 Cell Complete Medium is a ready-to-use medium that can be directly used for the culture of THP-1 cells, which is carefully designed and optimized by Pricella's R&D team based on RPMI-1640 basal medium. This high-quality complete medium has been strictly verified by THP-1 cells which could promote cell proliferation and maintain cell properties.

Manufacturer: Elabscience

IMDM (Iscove's Modified Dulbecco Medium) is an improved DMEM medium, which is used for the culture of erythrocyte progenitor cells and macrophages. The IMDM medium was supplemented with selenium, HEPES, sodium pyruvate and additional amino acids and vitamins on the basis of the DMEM medium, and potassium nitrate instead of iron nitrate, which is suitable for the rapid proliferation, high-density cell culture. IMDM medium can not only culture cells with special nutritional requirements (such as mouse B lymphocytes, stimulated B cells stimulated by LPS, bone marrow hematopoietic cells, T lymphocytes and various hybridoma cells), It can also be used as a base solution for some unique serum-free medium. This product contains many kinds of amino acids, vitamins, inorganic salts and other ingredients for cell culture, but does not contain protein, lipids or any growth factors. Therefore, the product should be used with serum or serum-free additives.

Manufacturer: Elabscience

DMEM (Dulbecco's Modified Eagle Medium) was developed on the basis of MEM medium. Compared with MEM medium, the content of amino acid increased by 2 times, the content of vitamin increased by 4 times, and the content of non-essential amino acid, trace iron ion and sodium pyruvate were increased by 4 times. The glucose content of DMEM medium was originally designed as 1000 mg/L (low Glucose type), and then developed into 4500 mg/L (high Glucoser type), which has been widely used in cell culture. DMEM medium without glucose can be added to the concentration of glucose at will according to the research needs, which is convenient and quick. DMEM (glucose free) contains many kinds of amino acids, vitamins, inorganic salts and other ingredients for cell culture, but does not contain protein, lipids or any growth factors, so the product should be used with serum or serum-free additives.

Manufacturer: Elabscience

Balanced Salt Solution (Physiological Solution) have the properties of buffer solution (regulate pH), normal saline (maintain osmotic pressure) and culture medium (provide nutrition). It can meet the basic needs of survival and metabolism of tissues, organs or cells in vitro. A small amount of phenolic red was added to some equilibrium salt solutions to indicate the pH change of the solution. Hank's Balanced Salt Solution (HBSS) is one of the commonly used phosphate buffers in cell separation or culture. The main components are NaCl、KCl、KH2PO4、Na2HPO4、NaHCO3 and glucose. HBSS is used for the washing of cells before passage and tissue dissociation, preparation of cell dissociation reagent, transport of cell or tissue, dilution of cells, etc.