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Manufacturer: Elabscience

Adenosine Triphosphate (ATP) is a high-energy phosphate compound, which is the most direct energy source in the body, and its content is directly related to the state of energy metabolism in the body. Hexokinase (HK) catalyzes the generation of glucose and ATP into glucose-6-phosphate, and glucose-6-phosphate dehydrogenase (G6PD) further catalyzes the dehydrogenation of glucose-6-phosphate into NADPH. NADPH can react with the color developer, and the product has a characteristic absorption peak at 460 nm to determine the ATP content.

Manufacturer: Elabscience

As an organelle, mitochondria is the "power factory" in cells and the main site of aerobic respiration of cells. Its function is to convert energy through oxidative phosphorylation to provide energy for cellular activities. The oxidation process is carried out by four respiratory chain membrane protein complexes (complexes I, II, III and IV) on the inner mitochondrial membrane. Mitochondrial complex III, also known as cytochrome c reductase complex, its main function is to oxidize the reduced coenzyme Q10 formed by mitochondrial complexes I and II to oxidative coenzyme Q10. In this process, the OD value increased at 550 nm. Therefore, the activity of mitochondrial complex III can be quantified by measure the change OD value at 550 nm.

Manufacturer: Elabscience

Oxaloacetic acid (OAA), one of the intermediates in the tricarboxylic acid cycle is an important substance in carbon and nitrogen metabolism. Oxaloacetate reacts with substrate acetyl-coA under the action of enzyme. The substance produced can react with DTNB, which has the maximum absorption peak at 412 nm. The content of oxaloacetate can be determined by measuring the absorbance value.

Manufacturer: Elabscience

Succinic acid, also known as succinic acid, is widely present in all plant and animal tissues, was first extracted from amber, is an intermediate in the citric acid cycle, and plays an important role in the production of energy in cells. Succinate, or succinate, is widely used in the agricultural, food and pharmaceutical industries due to its low toxicity. Succinic acid is catalyzed by enzyme reagent to form colored substance with color developer. The maximum absorption is at 555 nm. The content of succinic acid in samples can be calculated by measuring OD value and standard curve at 555 nm.

Manufacturer: Elabscience

L-malic acid, also known as 2-hydroxysuccinic acid, is widely present in living organisms. It has two stereoisomers and is one of the members of the tricarboxylic acid cycle, an important metabolic cycle in living organisms. The detection principle of this kit is that the enzyme catalyzes malic acid to produce oxaloacetic acid. At the same time, under the action of electronic coupler, the chromogenic agent is reduced to produce orange yellow product, which has the maximum absorption peak at about 450 nm.

Manufacturer: Elabscience

Coenzyme A (CoA) is composed of units derived from cysteine, pantothenic acid, and ATP. It plays an important role in the synthesis and oxidation of fatty acids, pyruvate oxidation in the citric acid cycle, and other biological processes. The enzymatic reaction of Coenzyme A produces NADH, and the chromogenic substance produced by reaction with the chromogenic agent has a characteristic absorption peak at 450 nm. The content of CoA in the sample can be determined by detecting the absorbance.

Manufacturer: Elabscience

Fumarase (FUM), also known as malate lyase, fumarate hydrase or fumarase, is an important enzyme in the tricarboxylic acid cycle, which can catalyze the hydration of fumarase to synthesize malic acid. FUM is a kind of intracellular enzyme that exists widely in plants and animals. The detection principle of this kit: fumarase catalyze fumaric acid products under the action of electronic coupler to reduce the chromogenic reagent to orange products, detection at about 450 nm has the maximum absorption peak.

Manufacturer: Elabscience

Phytic acid, also known as inositol hexaphosphate, is widely found in cereals, beans, and vegetables. The detection principle of this kit: Phytic acid generates inorganic phosphorus under the catalysis of enzyme. Under acidic conditions, inorganic phosphorus reacts with ammonium molybdate to produce blue molybdenum blue substance, which has a characteristic absorption peak at 700 nm. By measuring the content of inorganic phosphorus, the phytic acid content can be calculated.

Manufacturer: Elabscience

Triglycerides (TG) can be hydrolyzed by lipoprotein lipase into glycerol and free fatty acids. Glycerol produces glycerol-3-phosphate and ADP under the catalysis of glycerol kinase (GK). Glycerol-3-phosphate produces hydrogen peroxide under the action of glycerol phosphate oxidase (GPO). In the presence of 4-aminoantipyrine and phenol, hydrogen peroxide is catalyzed by peroxidase (POD) to produce quinones which is proportional to the content of TG.