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Manufacturer: Elabscience

Mitochondrial complex V is also known as F0F1-ATP synthase. ATP is hydrolyzed by F0F1-ATP synthase to produce ADP, and ADP converts NADH into oxidized coenzyme I (NAD) after enzyme conversion reaction. The activity of mitochondrial complex V can be quantified by measure the change OD value at 340 nm.

Manufacturer: Elabscience

Lipid peroxides (LPO) are substances produced by the action of reactive oxygen species (ROS) on unsaturated fatty acids. The LPO produced by the reaction will continue to oxidize the liposomes, thereby accumulating LPO in a chain reaction. Excess LPO decomposes into highly active aldehydes such as Acrolein, Malondialdehyde (MDA), and 4-hydroxy-2-nonenal (4-NHE), causing cell toxicity and cell death. This kit provides a fluorescent probe that can detect LPO production. This fluorescent probe can react with the lipid free radicals in the lipid peroxidation pathway, so as to detect the level of lipid peroxidation in cells. The probe fluoresces red under normal conditions through photoexcitation, and the fluorescence changes from red to green with the process of lipid peroxidation. Through the enhancement of green fluorescence intensity, the production of LPO can be detected with high sensitivity.

Manufacturer: Elabscience

Mitochondrial oxidative phosphorylation consumes oxygen to produce ATP, which provides energy for cell growth. Therefore, detection of cellular oxygen consumption is a key indicator of mitochondrial function. The kit provides a fluorescent probe, which is sensitive to oxygen. The fluorescence of the probe increases with the decrease of oxygen in a closed environment, and the oxygen consumption rate of cells is judged by detecting the change of the fluorescence value.

Manufacturer: Elabscience

Lipoxygenase (LOX) is an iron-containing oxidoreductase found in plants and animals. The detection principle of this kit is as follows: LOX catalyzes the oxidation of arachidonic acid to produce fluorescence products, and the enzyme activity of LOX can be calculated according to the change of fluorescence value of fluorescence products at the excitation wavelength of 507 nm and the emission wavelength of 547 nm within a unit time.

Manufacturer: Elabscience

Fatty acid oxidation (FAO) is the main pathway of fatty acid decomposition in the body, which can supply a large amount of energy required by the organism. FAO is also the transformation process of fatty acids. The length of fatty acid chain required by the organism is different. Through oxidation, long-chain fatty acids can be transformed into fatty acids with suitable growth degree for the organism metabolism. The detection principle of this kit: FAO process consumes the substrate and NAD+, and generates NADH. Under the action of electron coupling agent and chromogenesis agent, the orange red substance is generated, which can be detected at 450 nm.

Manufacturer: Elabscience

Acetyl-CoA is an essential cofactor and acyl carrier in the enzymatic acetyl transfer reaction. It can be formed by oxidative decarboxylation of pyruvate in mitochondria, oxidation of long-chain fatty acids, or oxidative degradation of certain amino acids. Acetyl-CoA is the starting compound of the citric acid cycle. The detection principle of this kit is that Acetyl-CoA is converted into other substances under the condition of enzyme catalysis, and the resulting fluorescence is detected by fluorescence microplate reader at the maximum excitation wavelength of 535 nm and the maximum emission wavelength of 587 nm.

Manufacturer: Elabscience

α-Ketoglutaric acid and alanine can be combined with fluorescent probe products under the action of a series of enzymes. The content of α-ketoglutaric acid in samples can be determined by measuring the fluorescence value.

Manufacturer: Elabscience

The probe in this kit has no fluorescence outside the cell. When it enters the cell, it will emit green fluorescence after hydrolysis by the intracellular ester. The fluorescence peaks at excitation wavelength 490 nm and emission wavelength 525 nm, and the fluorescence intensity is proportional to the calcium ion concentration.

Manufacturer: Elabscience

NADP-MDH catalyzes the conversion of malic acid and NADP+ to oxaloacetic acid and NADPH. NADPH makes WST-8 orange under the action of electron coupling agent. The activity of NADP-MDH can be calculated by measuring the change of absorbance value at 450 nm.