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Manufacturer: Elabscience

The Mitochondrial Complex I (NADH-CoQ Reductase) Activity Assay Kit (Catalog No. E-BC-K149-M) from Elabscience is designed to measure the activity of mitochondrial Complex I, also known as NADH-CoQ reductase, in animal tissue samples. This enzyme plays a crucial role in the mitochondrial electron transport chain, facilitating the transfer of electrons from NADH to ubiquinone (CoQ).

Manufacturer: Elabscience

The Citric Acid (CA) Colorimetric Assay Kit (Catalog No. E-BC-K351-S) from Elabscience is designed to measure citric acid content in various samples, including tissue, mitochondria, and other liquid samples. This assay is particularly useful for studying metabolic pathways and energy production processes.

Manufacturer: Elabscience

The Mitochondrial Complex IV (Cytochrome C Oxidase) Activity Assay Kit (Catalog No. E-BC-K152-M) from Elabscience is designed to measure the activity of mitochondrial Complex IV, also known as cytochrome C oxidase, in animal tissue samples. This enzyme is a key component of the mitochondrial respiratory chain, facilitating the transfer of electrons from cytochrome c to oxygen, thereby playing a crucial role in cellular energy production.

Manufacturer: Elabscience

The Mitochondrial Complex II Activity Assay Kit (Catalog No. E-BC-K150-M) from Elabscience is designed to measure the activity of mitochondrial Complex II, also known as succinate dehydrogenase, in animal tissue samples. This enzyme plays a crucial role in the citric acid cycle and the electron transport chain, contributing to cellular energy production.

Manufacturer: Elabscience

The Mitochondrial Complex III (Coenzyme Q-Cytochrome c Reductase) Activity Assay Kit (Catalog No. E-BC-K151-M) from Elabscience is designed to measure the activity of mitochondrial Complex III, also known as cytochrome c reductase. This enzyme plays a crucial role in the mitochondrial respiratory chain by facilitating the transfer of electrons from coenzyme Q to cytochrome c, contributing to cellular energy production.

Manufacturer: Elabscience

The Citric Acid (CA) Colorimetric Assay Kit (Catalog No. E-BC-K351-M) from Elabscience is designed to measure citric acid content in various samples, including tissues and mitochondria. This assay is essential for studying the citric acid cycle, a fundamental component of cellular energy metabolism.

Manufacturer: Elabscience

Malate dehydrogenase widely exists in animals, plants, bacteria and other organisms, is one of the key enzymes in tricarboxylic acid cycle, catalyze between malic acid and oxaloacetic acid reversible conversion. MDH can be divided into NAD-dependent MDH and NADP-dependent MDH according to different coenzyme specificity. NAD-MDH usually exists in the cytoplasm and mitochondria of bacteria and eukaryotic cells. NAD-MDH catalyzes the conversion of substrate malic acid and NAD to oxaloacetic acid and NADH. NADH makes WST-8 orange under the action of electron coupling agent. The activity of NAD-MDH can be calculated by measuring the change of absorbance value at 450 nm.

Manufacturer: Elabscience

SDH catalyzes the dehydrogenation of succinate to fumarate, with electron transport materials transferring electrons to 2, 6-dichlorophenol indiophenol (DCPIP). Then, the reduced DCPIP is reduced to the oxidized DCPIP, which has a characteristic absorption peak at 600 nm. Therefore, the activity of SDH can be quantified by measure the change OD value at 600 nm.

Manufacturer: Elabscience

Under the activation of the activator, IDH converts isocitrate into α-ketoglutaric acid. Meanwhile, NAD+ is reduced to NADH, which under the action of hydrogen transmitter, transfer electrons to WST-8 to produce the yellow product. The activity of NAD-IDH can be calculated by measuring the change of absorbance value at 450 nm.