Cell Culture

Manufacturer: Elabscience

RAW 264.7 Cell Complete Medium is a ready-to-use medium that can be directly used for the culture of RAW 264.7 cells, which is carefully designed and optimized by Pricella's R&D team based on DMEM basal medium. This high-quality complete medium has been strictly verified by RAW 264.7 cells which could promote cell proliferation and maintain cell properties.

Manufacturer: Elabscience

Penicillin-streptomycin solution mixture is the most commonly used antibiotic to prevent microbial contamination in vitro. Penicillin can interfere with the synthesis of bacterial cell wall, especially for Gram-positive bacteria. Streptomycin could bind to ribosomal ribosome for 30S and inhibit the synthesis of bacterial protein. It was effective for Gram-negative bacteria and Gram-positive bacteria, but especially for Gram-negative bacteria. The combined use of penicillin and streptomycin can prevent most bacterial contamination, but penicillin solution is sensitive to temperature and pH, easy to degrade at room temperature, and needs cryopreservation, the stability of penicillin solution is the most stable when pH is 6.0-6.5. Streptomycin was relatively stable, and pH 5.0-7.5 was the most stable.

Manufacturer: Elabscience

Trypsin is a serine hydrolase that can cut the base-side segment of the lysine and arginine residue in the polypeptide chain, hydrolyze the protein between the cells, and destroy the connection between the cells, so that the tissue or the adherent cells are dispersed into a single cell. The activity of the trypsin is related to the characteristics of the tissue or the cells, the concentration of the trypsin, the temperature and the time of action. At pH 8.0 and 37℃, the effect of the trypsin is the best. Therefore, the concentration of trypsin, the temperature and the time of the pancreatic enzyme should be grasped to avoid the excessive damage of the cells. In general, the frequently-used working concentration of the trypsin is 0.25%, while the concentration of trypsin for the semi-adherent cells or the cells is 0.05%. Since EDTA is able to bind Ca2+ and Mg2+ to destroy the cell connection to promote the dissociation of the cells, a certain amount of EDTA is often added to the trypsin solution to enhance the dissociation effect.

Manufacturer: Elabscience

McCoy's 5A medium was initially used specifically for the culture of Novikoff Hepatoma cells. Unlike other medium, McCoy's 5A medium contains reduced glutathione, bacterial peptone and high concentrations of glucose. McCoy's 5A is widely used in the culture of primary cells, such as bone marrow, skin, gingiva, kidney, spleen, lung, rat embryo, omentum and so on. In addition, McCoy's 5A medium is also used for tissue biopsy culture, the establishment of cell line, and the culture of some lymphocytes and more difficult cells. This product contains many kinds of amino acids, vitamins, inorganic salts and other ingredients for cell culture, but does not contain protein, lipids or any growth factors. Therefore, the product should be used with serum or serum-free additives.

Manufacturer: Elabscience

Balanced Salt Solution (Physiological Solution) have the properties of buffer solution (regulate pH), normal saline (maintain osmotic pressure) and culture medium (provide nutrition). It can meet the basic needs of survival and metabolism of tissues, organs or cells in vitro. A small amount of phenolic red was added to some equilibrium salt solutions to indicate the pH change of the solution. Dulbecco's Phosphate Buffered Saline (DPBS) is one of the most widely used equilibrium salt solutions in biochemistry. The main components are NaCl, KCl, KH2PO4 and Na2HPO4. DPBS can be divided into two types according to whether contain calcium and magnesium. Different from conventional PBS, DPBS phosphate content was slightly lower. DPBS was mainly used for embryological research.

Manufacturer: Elabscience

The DMEM/F12 medium is based on the DMEM medium, adding more nutritious elements from F12 medium, which contains a variety of trace elements, and is widely used in the culture of many mammalian cells. At the same time, DMEM/F12 medium is often used as the basis for the development of serum-free medium. It is also suitable for mammalian cell culture and clone density culture under low serum content. This product contains many kinds of amino acids, vitamins, inorganic salts and other ingredients for cell culture, but does not contain protein, lipids or any growth factors. Therefore, the product should be used with serum or serum-free additives.

Manufacturer: Elabscience

MEM medium (Minimum Essential Medium) was developed on the basis of Eagle basic medium. It is one of the most basic and widely used culture medium, and one of the most commonly used culture medium in animal cell culture. MEM medium contains 12 kinds of essential amino acids, glutamine and 8 vitamins, which is simple, mainly used in the culture of adherent cells. MEM, with NEAA medium is added L-alanine, L-glutamic acid, L-asparagine, L-aspartic acid, L-proline, L-serine and glycine on the basis of MEM medium. These 7 kinds of NEAA, can reduce the side effects of producing non-essential amino acids during cell culture and promote cell proliferation and metabolism. This product contains many kinds of amino acids, vitamins, inorganic salts and other ingredients for cell culture, but does not contain protein, lipids or any growth factors. Therefore, the product should be used with serum or serum-free additives.

Manufacturer: Elabscience

RPMI-1640 is an improved McCoy's 5A medium, which uses bicarbonate buffer system. RPMI-1640 medium was originally designed for lymphocyte culture. Nowadays, it has been widely used in the culture of normal cells and cancer cells, especially suspension cells, which is one of the most widely used media. RPMI-1640 medium contains many kinds of amino acids, vitamins, inorganic salts and other ingredients for cell culture, but does not contain protein, lipids or any growth factors. Therefore, the product should be used serum or serum-free additives.

Manufacturer: Elabscience

Balanced Salt Solution (Physiological Solution) have the properties of buffer solution (regulate pH), normal saline (maintain osmotic pressure) and culture medium (provide nutrition). It can meet the basic needs of survival and metabolism of tissues, organs or cells in vitro. A small amount of phenolic red was added to some equilibrium salt solutions to indicate the pH change of the solution. Phosphate Buffered Saline (PBS) is one of the most widely used equilibrium salt solutions in biochemistry. The main components are Na2HPO4、KH2PO4、NaCl and KCl. PBS buffers are often used to rinse tissue or cells, to transport cells or tissues, to prepare other reagents, to dilute the cells when counting the cells.